{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:28:15Z","timestamp":1777868895259,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme"],"abstract":"<jats:p>The subcellular repartition and the distinctive properties of guanylate cyclase (EC\n4.6.1.2) vary according to the lymphocyte population studied and according to the presence of\ndetergent. Guanylate cyclase of non-adherent peripheral lymphocytes and of thymus lymphocytes\nis recovered by more than 90% in the soluble fraction of the homogenate. Kinetics\naccording to the substrate (5'-GTP-Mn^2+) is Michaelian, the Ca^2+ ion acts as an activator,\nespecially in the case of blood lymphocytes, and the detergent has no effect on the enzyme\nactivity. On the other hand, the guanylate cyclase of tonsil lymphocytes reside in the particulate\nfraction. It has non-Michaelian kinetics for the substrate, a strong stimulating effect of\ndetergent, and an inhibitory effect of Ca^2+. A comparison of the enzymatic activities of unseparated\nand of non-adherent tonsil lymphocytes obtained from the same donor points to a\ncorrelation between their T and B properties: predominant soluble activity in the T population\nand particulate guanyl cyclase activity in the B subset.<\/jats:p>","DOI":"10.1159\/000469612","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:23:05Z","timestamp":1502472185000},"page":"86-92","source":"Crossref","is-referenced-by-count":5,"title":["Guanylate Cyclase Activity of Human Lymphocytes from\nPeripheral Blood, Thymus, and Tonsils"],"prefix":"10.1159","author":[{"given":"Y.","family":"Cille","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Deviller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Betuel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000469612","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:50:00Z","timestamp":1777553400000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000469612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000469612","archive":["Portico"],"relation":{},"ISSN":["0013-9432","2504-2564"],"issn-type":[{"value":"0013-9432","type":"print"},{"value":"2504-2564","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}