{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:28:18Z","timestamp":1777868898443,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme"],"abstract":"<jats:p>There is an iron-dependent activity in the venom of Crotalus atrox which\nappears to hydrolyze the N-glycosidic sugar-base bond of pyrimidine and purine nucleotides.\nMaximal activation of this activity occurred at 1.0 mmol\/1 and 0.8 mmol\/1 FeCl(3) for cytidine\ndiphosphate (CDP) and ADP substrates, respectively. The release of free base affects the\nbackground values of control experiments carried out during nucleotide-labelled assays of\nribonucleotide reductase which require the conversion of nucleotides to nucleosides by snake\nvenom treatment. When the reductase is examined in intact cells, a situation closely resembling\nnormal physiological conditions for the enzyme, FeCl(3) was found to be an inhibitor of\nADP reductions and varied from a mild stimulator to a significant inhibitor of CDP reductions\ndepending upon FeCl(3) concentration. Possible explanations for previously observed\nvariability in ribonucleotide reductase activity in the presence of iron are suggested.<\/jats:p>","DOI":"10.1159\/000469641","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:23:29Z","timestamp":1502472209000},"page":"223-228","source":"Crossref","is-referenced-by-count":0,"title":["An Iron-Dependent 5'-Nucleotide Phosphoribohydrolase\nActivity in the Venom of Crotalus atrox and Its Effect upon the\nDetermination of Mammalian Ribonucleotide Reductase"],"prefix":"10.1159","author":[{"given":"R.G.","family":"Hards","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.A.","family":"Wright","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000469641","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:50:06Z","timestamp":1777553406000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000469641"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000469641","archive":["Portico"],"relation":{},"ISSN":["0013-9432","2504-2564"],"issn-type":[{"value":"0013-9432","type":"print"},{"value":"2504-2564","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}