{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T06:46:27Z","timestamp":1781333187807,"version":"3.54.1"},"reference-count":18,"publisher":"S. Karger AG","issue":"5-6","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intervirology"],"published-print":{"date-parts":[[2016]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; Drug-resistant isolates of human cytomegalovirus (HCMV) have led to the development of new anti-HCMV drugs. Maribavir (MBV) is a novel inhibitor of the HCMV viral kinase. Resistance to MBV is mapped to gene UL27, a viral nuclear protein. In this study, we investigated UL27 polymorphisms in MBV-naive HIV-positive and HCMV congenitally infected clinical samples. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; DNA was extracted from 20 CMV-positive HIV (9\/20) and congenitally infected (11\/20) patients and used for UL27 polymerase chain reaction amplification. Sanger sequencing and multiple sequence alignment of products was performed. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; K90 was the most prevalent polymorphism in both HIV-positive and congenitally infected patients. Polymorphisms Q54, D123, and R107 (10%) were seen in more than one sample. There were significantly more polymorphisms in the HIV-positive samples (&lt;i&gt;p&lt;\/i&gt; = 0.038). &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; HCMV pUL27 is highly variable in adult immunocompromised HIV-positive patients.<\/jats:p>","DOI":"10.1159\/000471484","type":"journal-article","created":{"date-parts":[[2017,4,12]],"date-time":"2017-04-12T21:01:33Z","timestamp":1492030893000},"page":"262-266","source":"Crossref","is-referenced-by-count":7,"title":["Frequent pUL27 Variations in HIV-Infected Patients"],"prefix":"10.1159","volume":"59","author":[{"given":"Azam","family":"Mirarab","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2489-585X","authenticated-orcid":false,"given":"Alireza","family":"Mohebbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdolvahab","family":"Moradi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naeme","family":"Javid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Ali","family":"Vakili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alijan","family":"Tabarraei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2017,4,13]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00062-12"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1586\/14789072.2.6.881"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12002"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.574"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2015.01.003"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2006.07.010"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.13.7124-7130.2004"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2010.11.004"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.21.11499-11506.2003"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-3-18"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.01.006"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0000000000001257"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.7727\/wimj.2014.378"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02585-05"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcl.2012.12.008"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01317"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2011.08.019"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.2217\/fvl-2016-0089"}],"container-title":["Intervirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/471484","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/471484","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/471484","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:31:28Z","timestamp":1745429488000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000471484"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016]]},"references-count":18,"journal-issue":{"issue":"5-6","published-online":{"date-parts":[[2017,7,20]]}},"URL":"https:\/\/doi.org\/10.1159\/000471484","archive":["Portico"],"relation":{},"ISSN":["0300-5526","1423-0100"],"issn-type":[{"value":"0300-5526","type":"print"},{"value":"1423-0100","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016]]}}}