{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:20:34Z","timestamp":1777868434388,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme Protein"],"abstract":"<jats:p>Ornithine carbamoyltransferase (OCT), a urea cycle enzyme, is located almost exclusively\nin liver mitochondria. We designed a sensitive enzyme-linked immunosorbent\nassay (ELISA) of serum OCT protein using an antibody against purified bovine enzyme.\nOCT protein measured using this method showed a good correlation with OCT\nactivity (r = 0.961), and was much higher in patients with liver diseases than in the controls.\nMeasurements of serum OCT protein in 442 healthy blood donors gave the upper\nlimit of normal range of 23 ng Eq\/ml (equivalent to the bovine enzyme) for males, 8 ng\nEq\/ml for females, and 16 ng Eq\/ml for males plus females. The values differ significantly\nbetween the sexes and depending on ages. This ELISA system is expected to be\nused as a pertinent liver function test.<\/jats:p>","DOI":"10.1159\/000474964","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:21:00Z","timestamp":1502472060000},"page":"10-17","source":"Crossref","is-referenced-by-count":3,"title":["A Sensitive Enzyme-Linked Immunosorbent Assay\nof Serum Ornithine Carbamoyltransferase"],"prefix":"10.1159","author":[{"given":"Yoshiko","family":"Watanabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunsuke","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikiko","family":"Ozaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigetoshi","family":"Fujiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuo","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masataka","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme and Protein"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000474964","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:56:39Z","timestamp":1777550199000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000474964"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000474964","archive":["Portico"],"relation":{},"ISSN":["1019-6773","2504-2556"],"issn-type":[{"value":"1019-6773","type":"print"},{"value":"2504-2556","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}