{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:20:35Z","timestamp":1777868435975,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme Protein"],"abstract":"<jats:p>We developed a sensitive enzyme-linked immunosorbent assay (ELISA) for serum\nornithine carbamoyltransferase (OCT) protein, and examined serum OCT concentrations\nin patients with various liver diseases. OCT concentrations were markedly elevated\nin cases of hepatic encephalopathy, \u2018acute on chronic\u2019, and those with the acute\nphase of acute hepatitis, moderately in chronic hepatitis, liver cirrhosis, hepatocellular\ncarcinoma, primary biliary cirrhosis, and slightly in those with a fatty liver. High percentages\n(92-98%) of patients with chronic hepatitis, liver cirrhosis and hepatocellular carcinoma\nhad higher than normal concentrations of serum OCT protein. There was a close\ncorrelation with aspartate aminotransferase (AST) and alanine aminotransferase (ALT)\nactivities and moderate correlations with those of mitochondrial AST, glutamate dehydrogenase\nand y-glutamyltranspeptidase. The OCT\/ALT ratio was higher in patients\nwith liver cirrhosis than in those with chronic hepatitis (p &lt; 0.001), and was still higher in\ncases of hepatocellular carcinoma (p &lt; 0.05). In 2 patients with \u2018acute on chronic\u2019 disease,\nOCT concentrations decreased similarly with or more rapidly than AST or ALT\nactivities after admission. In 2 patients with hepatic encephalopathy, the OCT concentrations\nchanged similarly with AST and ALT activities. This OCT ELISA system will\naid in diagnosing various liver diseases and in the follow-up of the patients, and the\nOCT\/ALT ratio may serve for a differential diagnosis of liver diseases.<\/jats:p>","DOI":"10.1159\/000474965","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:21:00Z","timestamp":1502472060000},"page":"18-26","source":"Crossref","is-referenced-by-count":10,"title":["Clinical Evaluation of Serum Ornithine\nCarbamoyltransferase by Enzyme-Linked\nImmunosorbent Assay in Patients with\nLiver Diseases"],"prefix":"10.1159","author":[{"given":"Yoshiko","family":"Watanabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunsuke","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigetoshi","family":"Fujiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuo","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masataka","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme and Protein"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000474965","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:56:40Z","timestamp":1777550200000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000474965"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000474965","archive":["Portico"],"relation":{},"ISSN":["1019-6773","2504-2556"],"issn-type":[{"value":"1019-6773","type":"print"},{"value":"2504-2556","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}