{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:20:35Z","timestamp":1777868435976,"version":"3.51.4"},"reference-count":0,"publisher":"S. Karger AG","license":[{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T00:00:00Z","timestamp":1502409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Enzyme Protein"],"abstract":"<jats:p>Carbamoylphosphate synthetase I (CPS I), a urea cycle enzyme, is located almost\nexclusively in the mitochondria of hepatocytes. The enzyme is unique in that it constitutes\nabout 2-6% of total liver protein and is composed of a large subunit of 160 kD. We\ndeveloped a sensitive enzyme-linked immunosorbent assay (ELISA) for measurement\nof the enzyme in plasma using an antibody against the rat enzyme. In galactosamine-induced\nrat acute hepatitis, plasma concentration of CPS I that was 1-2 pg\/ml blood before\nthe treatment, increased up to 125 gg\/ml blood in 24 h after the treatment and\ndecreased to a near control level in 72 h. Plasma concentration of ornithine carbamoyltransferase\n(OCT), another urea cycle enzyme, reached a maximum in 24 h and then decreased\na little more rapidly than that of CPS I. On the other hand, alanine aminotransferase\nactivity reached a maximum in 36 h and decreased to a normal level in 96 h. In\nimmunoblot analysis, the native CPS I polypeptide of 160 kD and its fragments of 140\nand 125 kD were detected 24-48 h after the treatment. When purified rat CPS 1 and bovine\nOCT were injected intravenously into rats, the enzymes disappeared from blood\nroughly exponentially with apparent half-lives of about 67 and 18 min, respectively.\nDevelopment of an ELISA for human CPS I and determination of the serum enzyme in\nvarious liver diseases remain to be performed.<\/jats:p>","DOI":"10.1159\/000474991","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T17:31:44Z","timestamp":1502472704000},"page":"213-221","source":"Crossref","is-referenced-by-count":9,"title":["Enzyme-Linked Immunosorbent Assay of\nCarbamoylphosphate Synthetase I: Plasma\nEnzyme in Rat Experimental Hepatitis and\nIts Clearance"],"prefix":"10.1159","author":[{"given":"Mikiko","family":"Ozaki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazutoyo","family":"Terada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaki","family":"Kanazawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigetoshi","family":"Fujiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimio","family":"Tomita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masataka","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,8,11]]},"container-title":["Enzyme and Protein"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/article-pdf\/doi\/10.1159\/000474991","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:56:48Z","timestamp":1777550208000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000474991"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1159\/000474991","archive":["Portico"],"relation":{},"ISSN":["1019-6773","2504-2556"],"issn-type":[{"value":"1019-6773","type":"print"},{"value":"2504-2556","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,11]]}}}