{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T01:50:07Z","timestamp":1768355407803,"version":"3.49.0"},"reference-count":0,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2017,12,16]],"date-time":"2017-12-16T00:00:00Z","timestamp":1513382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2017,12,16]],"date-time":"2017-12-16T00:00:00Z","timestamp":1513382400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Innate Immun"],"published-print":{"date-parts":[[2018]]},"abstract":"Invasive aspergillosis mainly occurs in immunocompromised patients and is commonly caused by <i>Aspergillus fumigatus<\/i>, while <i>A.<\/i><i>nidulans<\/i> is rarely the causative agent. However, in chronic granulomatous disease (CGD) patients, <i>A. nidulans<\/i> is a frequent cause of invasive aspergillosis and is associated with higher mortality. Immune recognition of <i>A. nidulans <\/i>was compared to <i>A. fumigatus <\/i>to offer an insight into why <i>A. nidulans<\/i> infections are prevalent in CGD. Live cell imaging with J774A.1 macrophage-like cells and LC3-GFP-mCherry bone marrow-derived macrophages (BMDMs) revealed that phagocytosis of <i>A. nidulans <\/i>was slower compared to <i>A. fumigatus<\/i>. This difference could be attributed to slower migration of J774A.1 cells and a lower percentage of migrating BMDMs. In addition, delayed phagosome acidification and LC3-associated phagocytosis was observed with <i>A. nidulans<\/i>. Cytokine and oxidative burst measurements in human peripheral blood mononuclear cells revealed a lower oxidative burst upon challenge with <i>A. nidulans<\/i>. In contrast, <i>A. nidulans<\/i> induced significantly higher concentrations of cytokines. Collectively, our data demonstrate that <i>A. nidulans <\/i>is phagocytosed and processed at a slower rate compared to <i>A. fumigatus,<\/i> resulting in reduced fungal killing and increased germination of conidia. This slower rate of <i>A. nidulans<\/i> clearance may be permissive for overgrowth within certain immune settings.<\/jats:p>","DOI":"10.1159\/000484562","type":"journal-article","created":{"date-parts":[[2017,12,15]],"date-time":"2017-12-15T22:01:22Z","timestamp":1513375282000},"page":"145-160","source":"Crossref","is-referenced-by-count":16,"title":["Differential Kinetics of Aspergillus nidulans and Aspergillus fumigatus Phagocytosis"],"prefix":"10.1159","volume":"10","author":[{"given":"Mark S.","family":"Gresnigt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharina L.","family":"Becker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Floris","family":"Leenders","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Fernanda","family":"Alonso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques F.","family":"Meis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith M.","family":"Bain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars P.","family":"Erwig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank L.","family":"van de Veerdonk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2017,12,16]]},"container-title":["Journal of Innate Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/484562","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/484562","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:17:42Z","timestamp":1745425062000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000484562"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,16]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,3,20]]},"published-print":{"date-parts":[[2018,3,27]]}},"URL":"https:\/\/doi.org\/10.1159\/000484562","archive":["Portico"],"relation":{},"ISSN":["1662-811X","1662-8128"],"issn-type":[{"value":"1662-811X","type":"print"},{"value":"1662-8128","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,16]]}}}