{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T21:04:21Z","timestamp":1780693461301,"version":"3.54.1"},"reference-count":26,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nephron"],"published-print":{"date-parts":[[2018]]},"abstract":"<b><i>Background\/Aims:<\/i><\/b><i><\/i> Genetic cell ablation using the human diphtheria toxin receptor (hDTR) is a new strategy used for analysing cellular function. Diphtheria toxin (DT) is a cytotoxic protein that leaves mouse cells relatively unaffected, but upon binding to hDTR it ultimately leads to cell death. We used a podocyte-specific hDTR expressing (Pod-DTR) mouse to assess the anti-permeability and cyto-protective effects of the splice isoform vascular endothelial growth factor (VEGF-A<sub>165<\/sub>b). <b><i>Methods:<\/i><\/b><i><\/i> The Pod-DTR mouse was crossed with a mouse that over-expressed VEGF-A<sub>165<\/sub>b specifically in the podocytes (Neph-VEGF-A<sub>165<\/sub>b). Wild type (WT), Pod-DTR, Neph-VEGF-A<sub>165<\/sub>b and Pod-DTR X Neph-VEGF-A<sub>165<\/sub>b mice were treated with several doses of DT (1, 5, 100, and 1,000 ng\/g bodyweight). Urine was collected and the glomerular water permeability (L<sub>p<\/sub>A\/V<sub>i<\/sub>) was measured ex vivo after 14 days. Structural analysis and podocyte marker expression were also assessed. <b><i>Results:<\/i><\/b><i><\/i> Pod-DTR mice developed an increased glomerular L<sub>p<\/sub>A\/V<sub>i<\/sub> 14 days after administration of DT (all doses), which was prevented when the mice over-expressed VEGF-A<sub>165<\/sub>b. No major structural abnormalities, podocyte ablation or albuminuria was observed in Pod-DTR mice, indicating this to be a mild model of podocyte disease. However, a change in expression and localisation of nephrin within the podocytes was observed, indicating disruption of the slit diaphragm in the Pod-DTR mice. This was prevented in the Pod-DTR X Neph-VEGF-A<sub>165<\/sub>b mice. <b><i>Conclusion:<\/i><\/b><i><\/i> Although only a mild model of podocyte injury, over-expression of the anti-permeability VEGF-A<sub>165<\/sub>b isoform in the podocytes of Pod-DTR mice had a protective effect. Therefore, this study further highlights the therapeutic potential of VEGF-A<sub>165<\/sub>b in glomerular disease.<\/jats:p>","DOI":"10.1159\/000485664","type":"journal-article","created":{"date-parts":[[2018,1,26]],"date-time":"2018-01-26T22:01:18Z","timestamp":1517004078000},"page":"51-62","source":"Crossref","is-referenced-by-count":6,"title":["Vascular Endothelial Growth Factor-A<sub>165<\/sub>b Restores Normal Glomerular Water Permeability in a Diphtheria-Toxin Mouse Model of Glomerular Injury"],"prefix":"10.1159","volume":"139","author":[{"given":"Megan","family":"Stevens","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher\u00a0R.","family":"Neal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew\u00a0H.J.","family":"Salmon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David\u00a0O.","family":"Bates","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven\u00a0J.","family":"Harper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Oltean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2018,1,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10072"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/90795"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.3.1015"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfg438"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2011040396"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005010055"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2012.133"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.90519.2008"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.4030.1"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00621.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2015080908"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159\/000177614"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00410.2011"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2014040350"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-6577"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009060617"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0707330"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.091146"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.099184"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1998.00219.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1981.121"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2016.3516"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1159\/000177614"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1113\/JP274481"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1172\/JCI17423"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2015.162"}],"container-title":["Nephron"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/485664","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/485664","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:26:55Z","timestamp":1745425615000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000485664"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":26,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018,4,26]]},"published-print":{"date-parts":[[2018,5,3]]}},"URL":"https:\/\/doi.org\/10.1159\/000485664","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.5827368","asserted-by":"subject"}]},"ISSN":["1660-8151","2235-3186"],"issn-type":[{"value":"1660-8151","type":"print"},{"value":"2235-3186","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}