{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T09:25:39Z","timestamp":1759742739229,"version":"3.40.4"},"reference-count":20,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathobiology"],"published-print":{"date-parts":[[2018]]},"abstract":"<b><i>Objective:<\/i><\/b> The aim of this study was to identify differences in proteome profiles of diffuse large B-cell lymphoma (DLBCL) of nongerminal center (non-GC) versus GC type in the search for new markers and drug targets. <b><i>Methods:<\/i><\/b> Six DLBCL, with 3 repeats for each, were used for the initial study by proteomics: 3 non-GC and 3 GC DLBCL cases. For immunohistochemistry, tissue microarrays were made from 31 DLBCL samples: 16 non-GC de novo lymphomas and 15 GC cases (11 transformed from follicular lymphomas and 4 de novo GC lymphomas). Proteome profiling was performed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. <b><i>Results:<\/i><\/b> Ninety-one proteins were found differentially expressed in non-GC compared to GC type. The Cytoscape tool was used for systemic analysis of proteomics data, revealing 19 subnetworks representing functions affected in non-GC versus GC types of DLBCL. <b><i>Conclusion:<\/i><\/b> A validation study of 3 selected proteins (BiP\/Grp78, Hsp90, and cyclin B2) showed the enhanced expression in non-GC DLBCL, supporting the proteomics data.<\/jats:p>","DOI":"10.1159\/000486285","type":"journal-article","created":{"date-parts":[[2018,4,4]],"date-time":"2018-04-04T21:01:18Z","timestamp":1522875678000},"page":"211-219","source":"Crossref","is-referenced-by-count":7,"title":["Proteomic Profiling of Diffuse Large B-Cell Lymphomas"],"prefix":"10.1159","volume":"85","author":[{"given":"Anna","family":"Kwieci\u0144ska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Porwit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nazariy","family":"Souchelnytskyi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann","family":"Kaufeldt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catharina","family":"Larsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svetlana","family":"Bajalica-Lagercrantz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serhiy\u00a0","family":"Souchelnytskyi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2018,4,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/35000501"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012914"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.83"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1545"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-05-577189"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038\/nature01514"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/260348"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.015362"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M115.050245"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/841769"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2004.05.003"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.07.031"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-13-1"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2014.03.035"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2014-202722"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2014.995647"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1172\/JCI26471"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-04-278853"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2012.05.024"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0000000000000089"}],"container-title":["Pathobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/486285","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/486285","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:32:20Z","timestamp":1745425940000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000486285"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":20,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,8,29]]},"published-print":{"date-parts":[[2018,9,4]]}},"URL":"https:\/\/doi.org\/10.1159\/000486285","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.6085949","asserted-by":"subject"}]},"ISSN":["1015-2008","1423-0291"],"issn-type":[{"type":"print","value":"1015-2008"},{"type":"electronic","value":"1423-0291"}],"subject":[],"published":{"date-parts":[[2018]]}}}