{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T10:26:44Z","timestamp":1770892004497,"version":"3.50.1"},"reference-count":29,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Nephrol"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt; Podocyte injury is a hallmark of minimal change disease (MCD). Calcineurin inhibitors have been widely used in the current treatment of MCD, and miR-499 may target calcineurin. We aimed to study the function of miR-499 in MCD and test whether miR-499 delivery can improve MCD. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; An MCD mouse model was generated using puromycin aminonucleoside (PAN). MiR-499 was delivered using lentiviruses. Biochemical indicators including serum albumin, triglyceride, cholesterol, and 24-h urine protein were determined. Targets of miR-499 were confirmed using reporter gene activity assays. The ultrastructure of podocytes was analyzed using transmission electron microscopy. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; MiR-499 significantly improved MCD-related symptoms and signs. Foot-process effacement was caused by PAN and partially reversed by miR-499. We identified that both CnA\u03b1 and CnA\u03b2 were targets of miR-499, and were overexpressed in the presence of PAN. However, miR-499 reduced the expression of CnA\u03b1 and CnA\u03b2, leading to a decreased activity of calcineurin signaling in mouse podocytes in vitro and in vivo. In addition, miR-499 recovered PAN-induced reduction of cell viability. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; MiR-499 ameliorated podocyte injury by targeting CnA\u03b1 and CnA\u03b2 in a PAN-induced MCD mouse model. Delivery of miR-499 can be a novel strategy for MCD treatment.<\/jats:p>","DOI":"10.1159\/000486967","type":"journal-article","created":{"date-parts":[[2018,2,15]],"date-time":"2018-02-15T22:01:31Z","timestamp":1518732091000},"page":"94-102","source":"Crossref","is-referenced-by-count":12,"title":["miR-499 Ameliorates Podocyte Injury by Targeting Calcineurin in Minimal Change Disease"],"prefix":"10.1159","volume":"47","author":[{"given":"Kaiyue","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjuan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuefang","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jidong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Hong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2018,2,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.05000516"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2012070734"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.7300.1"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfw021"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfq617"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0000000000000234"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1102703"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2000.80.4.1483"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038\/srep32087"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1857"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.4103\/0971-4065.107197"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2016.246"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.201606372"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.265736"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2282"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.05005"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S118391"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00699-2"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-016-2109-4"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks466"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0155504"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63430-X"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000144207.44469.BE"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2011.07.024"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1007\/s12079-012-0184-0"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1111\/nep.12256"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00964.x"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1159\/000089689"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006070710"}],"container-title":["American Journal of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/486967","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/486967","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:39:11Z","timestamp":1745426351000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000486967"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":29,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,3,14]]},"published-print":{"date-parts":[[2018,3,20]]}},"URL":"https:\/\/doi.org\/10.1159\/000486967","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.5891401","asserted-by":"subject"}]},"ISSN":["0250-8095","1421-9670"],"issn-type":[{"value":"0250-8095","type":"print"},{"value":"1421-9670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}