{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T20:00:06Z","timestamp":1760385606488,"version":"3.40.4"},"reference-count":23,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"published-print":{"date-parts":[[2018]]},"abstract":"<b><i>Background:<\/i><\/b> Premature birth is a significant health care burden. Xenon (Xe) is a general anesthetic with neuroprotective effects. <b><i>Objectives:<\/i><\/b> Here, we investigate the neuroprotective role of Xe in a lipopolysaccharide (LPS)- and hypoxia-ischemia (HI)-induced white matter damage (WMD) model. <b><i>Methods:<\/i><\/b> Three-day-old Sprague-Dawley rats were randomly divided into a sham group (group A, <i>n<\/i> = 24), an LPS + HI group (group B, <i>n<\/i> = 24), and an LPS + HI + Xe group (group C, <i>n<\/i> = 72). The onset of Xe inhalation started at 0, 2, and 5 h in subgroups C1, C2, and C3, respectively. Next, we performed TUNEL and hematoxylin and eosin (HE) staining; and examined the expression of CLIC4 and Bcl-2 in brain tissues. <b><i>Results:<\/i><\/b> HE staining revealed distorted cytoarchitecture, tangled nerve fibers, and pyknosis in group B, while Xe treatment improved these histological alterations in the group C pups. Following LPS and HI insult, the number of apoptotic cells significantly increased in group B at 48 and 72 h (<i>p<\/i> &#x3c; 0.05), and Xe significantly alleviated apoptosis (<i>p<\/i> &#x3c; 0.001) at 24, 48, and 72 h, respectively. Similarly, CLIC4 mRNA expression was significantly increased in group B (<i>p<\/i> &#x3c; 0.05), and Xe produced a marked reduction in CLIC4 mRNA expression in group C subgroups (<i>p<\/i> &#x3c; 0.05). Western blotting demonstrated enhanced Bcl-2 expression in group C when compared to group B (<i>p<\/i> &#x3c; 0.05). <b><i>Conclusions:<\/i><\/b> These results demonstrate that LPS and HI successfully induced WMD, and Xe decreased neuronal apoptosis via Bcl-2- and CLIC4-mediated pathways. Moreover, the therapeutic time window of Xe extended for up to 5 h. These findings suggest that Xe can be used as a protective treatment for WMD in premature infants.<\/jats:p>","DOI":"10.1159\/000487220","type":"journal-article","created":{"date-parts":[[2018,3,8]],"date-time":"2018-03-08T22:01:25Z","timestamp":1520546485000},"page":"339-346","source":"Crossref","is-referenced-by-count":8,"title":["Impact of Xenon on CLIC4 and Bcl-2 Expression in Lipopolysaccharide and Hypoxia-Ischemia-Induced Periventricular White Matter Damage"],"prefix":"10.1159","volume":"113","author":[{"given":"Xiangyun","family":"Yin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jixiu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangliang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmin","family":"Xi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangli","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohang","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaotong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lulu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2018,3,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2006-1522"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2014-0898"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600184"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008070712"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1213\/ane.Ob013e31818874bf"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2013-303786"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e3181cea398"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s00101-015-0079-6"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.4161\/auto.6260"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M509349200"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.3892\/or.2013.2265"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-014-0215-2"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-9420com"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.22617"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1001\/jama.292.19.2357"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1159\/000362782"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e3181df5f6b"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-9-175"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410160102"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2009-1244"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0000000000000624"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037020"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1097\/EJA.0b013e3283583c4b"}],"container-title":["Neonatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/487220","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/487220","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:41:33Z","timestamp":1745426493000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000487220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":23,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,5,31]]},"published-print":{"date-parts":[[2018,5,29]]}},"URL":"https:\/\/doi.org\/10.1159\/000487220","archive":["Portico"],"relation":{},"ISSN":["1661-7800","1661-7819"],"issn-type":[{"type":"print","value":"1661-7800"},{"type":"electronic","value":"1661-7819"}],"subject":[],"published":{"date-parts":[[2018]]}}}