{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T12:11:56Z","timestamp":1769602316452,"version":"3.49.0"},"reference-count":0,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; Our objective was to verify the sensitivity and specificity of dual immunocytochemistry staining for p16 and Ki-67 in liquid-based samples (the \u201cdual\u201d assay) for cervical lesion screening, compared to biopsy findings and human papillomavirus (HPV) DNA molecular detection. &lt;b&gt;&lt;i&gt;Study Design:&lt;\/i&gt;&lt;\/b&gt; Sensitivity, specificity, and positive (PPV) and negative (NPV) predictive values for the \u201cdual immunocytochemistry assay\u201d were calculated and compared to histopathological results and to high-risk HPV DNA detection in adult women or teenagers submitted to cervical cancer screening. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; A total of 151 women were included. The majority (96.2%) of those with negative dual assay results had lower biopsy grades (&lt;i&gt;p&lt;\/i&gt; &amp;#x3c; 0.001). Women with cytology results suggestive of cervical cancer had positive dual immunocytochemistry assay results more frequently (&lt;i&gt;p&lt;\/i&gt; &amp;#x3c; 0.001), and these positive results were also significantly associated with biopsy findings (&lt;i&gt;p&lt;\/i&gt; &amp;#x3c; 0.001) and with high-risk genotype HPV infection (&lt;i&gt;p&lt;\/i&gt; = 0.007). Specificity and PPV for the dual assay were 0.972 (0.855\u20130.999) and 0.800 (0.284\u20130.995), respectively, and 1.000 (0.590\u20131.000) and 1.000 (0.631\u20131.000) for HPV detection. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; The dual immunocytochemistry assay had high specificity and PPV. It reveals a persistent HPV infection, avoiding the need for new tissue collections for biopsies or hybrid capture.<\/jats:p>","DOI":"10.1159\/000487504","type":"journal-article","created":{"date-parts":[[2018,3,22]],"date-time":"2018-03-22T22:01:17Z","timestamp":1521756077000},"page":"104-114","source":"Crossref","is-referenced-by-count":10,"title":["Dual p16 and Ki-67 Expression in Liquid-Based Cervical Cytological Samples Compared to Pap Cytology Findings, Biopsies, and HPV Testing in Cervical Cancer Screening: A Diagnostic Accuracy Study"],"prefix":"10.1159","volume":"62","author":[{"given":"Karla\u00a0Cala\u00e7a\u00a0Kabbach","family":"Prigenzi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tha\u00eds\u00a0","family":"Heinke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael\u00a0Calil","family":"Salim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo\u00a0Rubino\u00a0de\u00a0Azevedo","family":"Focchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2018,3,22]]},"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/487504","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/487504","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:43:47Z","timestamp":1745426627000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000487504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,4,20]]},"published-print":{"date-parts":[[2018,4,26]]}},"URL":"https:\/\/doi.org\/10.1159\/000487504","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"value":"0001-5547","type":"print"},{"value":"1938-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}