{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T20:59:22Z","timestamp":1777409962185,"version":"3.51.4"},"reference-count":31,"publisher":"S. Karger AG","issue":"1-2","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur Surg Res"],"published-print":{"date-parts":[[2018]]},"abstract":"<b><i>Background\/Purpose:<\/i><\/b> Calcifications and absence of growth potential are the major drawbacks of glutaraldehyde-treated prosthesis. Decellularized and secured xeno-\/allogeneic matrices were assessed in a preclinical porcine model for biocompatibility and vascular remodeling in comparison to glutaraldehyde-fixed bovine pericardium (GBP; control). <b><i>Methods:<\/i><\/b> Native human (fascia lata, pericardium) and porcine tissues (peritoneum) were used and treated. In vitro, biopsies were performed before and after treatment to assess decellularization (hematoxylin and eosin\/DAPI). In vivo, each decellularized and control tissue sample was implanted subcutaneously in 4 mini-pigs. In addition, 9 mini-pigs received a patch or a tubularized prosthesis interposition on the carotid artery or abdominal aorta of decellularized (D) human fascia lata (DHFL; <i>n<\/i> = 4), human pericardium (DHP; <i>n<\/i> = 9), porcine peritoneum (DPPt; <i>n<\/i> = 7), and control tissue (GBP: <i>n<\/i> = 3). Arteries were harvested after 1 month and subcutaneous samples after 15\u201330 days. Tissues were processed for hematoxylin and eosin\/von Kossa staining and immunohistochemistry for CD31, alpha-smooth muscle actin, CD3, and CD68. Histomorphometry was achieved by point counting. <b><i>Results:<\/i><\/b> A 95% decellularization was confirmed for DHP and DPPt, and to a lower degree for DHFL. In the subcutaneous protocol, CD3 infiltration was significantly higher at day 30 in GBP and DHFL, and CD68 infiltration was significantly higher for GBP (<i>p<\/i> &#x3c; 0.05). In intravascular study, no deaths, aneurysms, or pseudoaneurysms were observed. Inflammatory reaction was significantly higher for DHFL and GBP (<i>p<\/i> &#x3c; 0.05), while it was lower and comparable for DHP\/DPPt. DHP and DPPt showed deeper recellularization, and a new arterial wall was characterized. <b><i>Conclusions:<\/i><\/b> In a preclinical model, DPPt and DHP offered better results than conventional commercialized GBP for biocompatibility and vascular remodeling.<\/jats:p>","DOI":"10.1159\/000487591","type":"journal-article","created":{"date-parts":[[2018,4,5]],"date-time":"2018-04-05T22:05:24Z","timestamp":1522965924000},"page":"58-71","source":"Crossref","is-referenced-by-count":13,"title":["Enhanced Vascular Biocompatibility and Remodeling of Decellularized and Secured Xenogeneic\/Allogeneic Matrices in a Porcine 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