{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:53:09Z","timestamp":1792029189978,"version":"4.4.0"},"reference-count":40,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microb Physiol"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>Spinosad, a member of polyketide-derived macrolides produced in the actinomycete &lt;i&gt;Saccharopolyspora spinosa&lt;\/i&gt;, has been developed as a broad-spectrum and effective insecticide. The \u03b2-oxidation pathway could be an important source of building blocks for the biosynthesis of spinosad, thus the effect of vegetable oils on the production of spinosad in a high-yield strain was investigated. The spinosad production increased significantly with the addition of strawberry seed oil (511.64 mg\/L) and camellia oil (520.07 mg\/L) compared to the control group without oil (285.76 mg\/L) and soybean oil group (398.11 mg\/L). It also revealed that the addition of oils would affect the expression of genes involved in fatty acid metabolism, precursor supply, and oxidative stress. The genetically engineered strain, in which &lt;i&gt;fadD1&lt;\/i&gt; and &lt;i&gt;fadE&lt;\/i&gt; genes of &lt;i&gt;Streptomyces coelicolor&lt;\/i&gt; were inserted, produced spinosad up to 784.72 mg\/L in the medium containing camellia oil, while a higher spinosad production level (843.40 mg\/L) was detected with the addition of 0.01 mM of thiourea.<\/jats:p>","DOI":"10.1159\/000487854","type":"journal-article","created":{"date-parts":[[2018,5,4]],"date-time":"2018-05-04T21:01:25Z","timestamp":1525467685000},"page":"53-64","source":"Crossref","is-referenced-by-count":10,"title":["Improvement of Spinosad Production upon Utilization of Oils and Manipulation of \u03b2-Oxidation in a High-Producing &lt;b&gt;&lt;i&gt;Saccharopolyspora spinosa&lt;\/i&gt;&lt;\/b&gt; 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