{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:28:39Z","timestamp":1745468919776,"version":"3.40.4"},"reference-count":22,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemotherapy"],"published-print":{"date-parts":[[2018]]},"abstract":"<b><i>Background:<\/i><\/b> <i>Chlamydia<\/i> is a group of bacterial pathogens distributed worldwide that can lead to serious reproductive and other health problems. The rise of antibiotic-resistant pathogens promotes the development of novel antichlamydial agents. The aim of this study is to assess in vitro antichlamydial activity of our previously synthesized 1,2,3,5- tetrasubstituted pyrroles. <b><i>Methods:<\/i><\/b> The derivatives were screened for their antichlamydial activity against three <i>Chlamydia<\/i> strains by calculating IC<sub>50<\/sub> values using concentration-response inhibition data between 1 and 32 \u03bcM. The action of the compounds on <i>Chlamydia<\/i> elementary body (EB) infectivity and the impact of the chemicals\u2019 administration time on their antichlamydial effect were evaluated to reveal the inhibitory mechanism. <b><i>Results:<\/i><\/b> Some of the compounds moderately inhibited the <i>Chlamydia<\/i> strains. Compound 10 exhibited the strongest inhibitory activity, with IC<sub>50<\/sub> values from 4.34 to 5.83 \u03bcM. These pyrrole derivatives inhibited <i>Chlamydia<\/i> infection by reducing EB infectivity during the early stage and disturbing <i>Chlamydia<\/i> growth by targeting the early-to-middle stage prior to 12 h of the chlamydial life cycle. <b><i>Conclusion:<\/i><\/b> Our findings highlight the potential of 1,2,3,5-tetrasubstituted pyrrole derivatives as promising lead molecules for the development of antichlamydial agents.<\/jats:p>","DOI":"10.1159\/000487880","type":"journal-article","created":{"date-parts":[[2018,4,12]],"date-time":"2018-04-12T21:01:35Z","timestamp":1523566895000},"page":"95-99","source":"Crossref","is-referenced-by-count":1,"title":["In vitro Antichlamydial Activity of 1,2,3,5-Tetrasubstituted Pyrrole Derivatives"],"prefix":"10.1159","volume":"63","author":[{"given":"Hongzhang","family":"Ji","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changyue","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Ni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nannan","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingyan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengju","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofeng","family":"Bao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2018,4,12]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2005.03.002"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.30"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2003.00352.x"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2010.163"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00106-07"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703218104"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.10.96"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ771"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.4.1843-1855.2004"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1586\/erv.11.139"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01677-14"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b01759"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1055\/s-0035-1545721"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1055\/s-0043-100382"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.201100430"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2015.01.014"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2015.12.017"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2014.06.075"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.201500350"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1021\/jm030266r"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1021\/jm010533y"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1021\/jp108254g"}],"container-title":["Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/487880","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/487880","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:47:07Z","timestamp":1745426827000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000487880"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":22,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,5,15]]},"published-print":{"date-parts":[[2018,5,24]]}},"URL":"https:\/\/doi.org\/10.1159\/000487880","archive":["Portico"],"relation":{},"ISSN":["0009-3157","1421-9794"],"issn-type":[{"type":"print","value":"0009-3157"},{"type":"electronic","value":"1421-9794"}],"subject":[],"published":{"date-parts":[[2018]]}}}