{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:18:31Z","timestamp":1773101911483,"version":"3.50.1"},"reference-count":27,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ophthalmic Res"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Purpose:&lt;\/i&gt;&lt;\/b&gt; Studying genipin variable concentrations, treatment durations, and delivery methods as a substance to increase corneal stiffness by inducing corneal collagen cross-linking (CXL). &lt;b&gt;&lt;i&gt;Materials and Methods:&lt;\/i&gt;&lt;\/b&gt; 100 bovine corneas treated with different genipin concentrations (0.1, 0.5, and 1%) and treatment durations (15 min, 40 min, 2 h, and 3 days) through different delivery methods compared to 10 controls treated with riboflavin\/UV. Histology examination, enzymatic digestion with collagenase and thermal differential scanning calorimetry were performed on the different samples. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Bovine corneas soaked in 0.5% genipin morphologically showed 4.7% CXL in comparison to 5.6% in controls (&lt;i&gt;p&lt;\/i&gt; &amp;#x3c; 0.05). Corneas treated with topical 0.5% genipin, by a 140-\u00b5L drop applied hourly for 2 h, showed 7% corneal CXL. Corneas treated with topical genipin 0.5% for 30 min, 1 and 2 h showed 54 \u00b1 6, 40 \u00b1 7, and 39 \u00b1 9% enzymatic degradation, respectively, in comparison to controls (74%). Corneas treated with 0.5% genipin for 1, 2, and 8 h showed higher thermal denaturation resistance (Td values of 64.9 \u00b1 0.3, 64.7 \u00b1 0.0 and 67.3 \u00b1 0.9), respectively, in comparison to the control group (64.6 \u00b1 0.5) (&lt;i&gt;p&lt;\/i&gt; &amp;#x3c; 0.05). &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; Genipin 0.5%, in a 140-\u00b5L drop applied hourly for 2 h, showed better potential to enhance corneal stiffness and stability through inducing CXL.<\/jats:p>","DOI":"10.1159\/000487950","type":"journal-article","created":{"date-parts":[[2018,5,25]],"date-time":"2018-05-25T21:01:19Z","timestamp":1527282079000},"page":"100-108","source":"Crossref","is-referenced-by-count":4,"title":["Optimizing Genipin Concentration for Corneal Collagen Cross-Linking: An ex vivo Study"],"prefix":"10.1159","volume":"60","author":[{"given":"Almutez\u00a0M.","family":"Gharaibeh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia","family":"Saez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nerea","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurent","family":"Bataille","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge\u00a0L.","family":"Ali\u00f3","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2018,5,25]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.3928\/1081597X-20160428-02"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2015.08.023"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.3109\/08820538.2014.962176"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038\/sj.eye.6700751"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e31818225c3"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(02)02220-1"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2004.11.007"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2003.12.011"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1067\/mtc.2001.117624"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002\/pola.20669"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2012.06.010"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2016.04.025"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056330"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(96)81413-X"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.3928\/1081597X-20140429-02"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-4328(03)00081-7"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.10476"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(01)00379-9"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2004.06.033"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1159\/000096231"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2006.05.015"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2015-308087"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2009.11.003"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4636(200010)52:1<58::AID-JBM8>3.0.CO;2-0"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(199911)47:2<116::AID-JBM2>3.0.CO;2-J"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.3791\/53119"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2008.11.009"}],"container-title":["Ophthalmic Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/487950","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/487950","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:48:07Z","timestamp":1745426887000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000487950"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":27,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,7,27]]},"published-print":{"date-parts":[[2018,8,2]]}},"URL":"https:\/\/doi.org\/10.1159\/000487950","archive":["Portico"],"relation":{},"ISSN":["0030-3747","1423-0259"],"issn-type":[{"value":"0030-3747","type":"print"},{"value":"1423-0259","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}