{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T10:57:58Z","timestamp":1784804278097,"version":"3.55.0"},"reference-count":44,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathobiology"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>Objectives: Scirrhous gastric cancers grow rapidly, and frequently invade the peritoneum. Such peritoneal dissemination properties markedly reduce patient survival. Thus, an effective means for inhibiting peritoneal dissemination is urgently required. Methods: We previously established a cell line, HSC-58, from a scirrhous gastric cancer patient, and further successfully isolated a metastatic line, 58As9, in nude mice upon orthotopic inoculation. Using the lines, we examined the mechanism underlying peritoneal dissemination from the viewpoint of microRNA (miRNA) expression. Results: miRNA array and qRT-PCR analysis showed that the expressions of epithelial-mesenchymal transition (EMT)-associated miRNAs such as miR-200c and miR-141 were significantly low in 58As9. Using 58As9 with stably overexpressing miR-200c, miR-141, or both, together with a luciferase reporter assay, we found that miR-200c targeted zinc finger E-box-binding homeobox 1 (ZEB1) and miR-141 targeted ZEB2. The overexpressed lines reversed the EMT status from mesenchymal to epithelial in 58As9, and significantly reduced the invasion activity and peritoneal dissemination for a significant prolongation of survival in the orthotopic tumor models in nude mice. Conclusions: EMT-associated miRNAs such as miR-200c and miR-141 and their target genes ZEB1\/ZEB2 have good potential for antiperitoneal dissemination therapy in patients with scirrhous gastric cancers.<\/jats:p>","DOI":"10.1159\/000488801","type":"journal-article","created":{"date-parts":[[2018,5,30]],"date-time":"2018-05-30T21:01:22Z","timestamp":1527714082000},"page":"232-246","source":"Crossref","is-referenced-by-count":5,"title":["MicroRNAs Associated with Epithelial-Mesenchymal Transition Can Be Targeted to Inhibit Peritoneal Dissemination of Human Scirrhous Gastric Cancers"],"prefix":"10.1159","volume":"85","author":[{"given":"Yoshifumi","family":"Takei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guodong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayami","family":"Morita-Kondo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshifumi","family":"Hara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keichiro","family":"Mihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuyoshi","family":"Yanagihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2018,5,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1835"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.11.007"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1172\/JCI39104"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038\/nrc822"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3447"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3758"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1126\/science.1203543"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s00595-008-3860-1"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1053\/ejso.1999.0780"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2004.tb02489.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2005.00054.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041312"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-0800"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038\/nature02871"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2131"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-2530"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1186\/2193-1801-3-288"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24382"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1089\/nat.2014.0526"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mt.6300360"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2011.221"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112100200"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20781"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.22068"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.4155\/tde.14.8"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1722"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1640608"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C800074200"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1038\/embor.2008.74"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1242\/dev.007047"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2009.207"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.4037"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-013-9456-2"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1002\/jso.22142"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.3892\/mco.2014.462"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1002\/jso.23564"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2014.2680"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-012-2042-6"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2015.177"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-3437-8"},{"key":"ref41","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2008.50"},{"key":"ref42","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201202014"},{"key":"ref43","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3820"},{"key":"ref44","doi-asserted-by":"publisher","DOI":"10.1177\/0300060513489789"}],"container-title":["Pathobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/pat\/article-pdf\/85\/4\/232\/3907298\/000488801.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/pat\/article-pdf\/85\/4\/232\/3907298\/000488801.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:22:47Z","timestamp":1745425367000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000488801"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"refe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