{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T06:49:48Z","timestamp":1782542988780,"version":"3.54.5"},"reference-count":13,"publisher":"S. Karger AG","issue":"5-6","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; To test the performance of a new fixative for pap smear collection for liquid-based cervical cytology, CellPreserv\u00ae and compare it with the commercially available, PreservCyt\u00ae used in the diagnosis and detection of human papillomavirus (HPV). &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Seven hundred twenty five women participated in this study after signing an informed consent. The specimens were collected using a traditional device, agitated in PBS, and equally divided in both fixatives. The slides were prepared routinely, stained by Papanicolaou, examined blindly by 2 cytologists, and reviewed by one cytopathologist. To search for HPV, 1,000 \u03bcL from each fixative was taken and processed by polymerase chain reaction. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Considering the adequacy of samples, both fixatives had similar results \u2013 0.33 and 0.32% of the cases unsatisfactory for PreservCyt\u00ae and CellPreserv\u00ae, respectively. Considering the 701 satisfactory cases and comparing the new fixative to the traditional fixative, there was 99.3% concordance between both. The results regarding the HPV detection was 100% concordant between the 2 fixatives. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; The new methanol-based fixative, CellPreserv\u00ae, is cheaper and equally efficient for treating cervical cancer screening and for HPV detection, and can be safely used by the health system prevailing in low-income countries.<\/jats:p>","DOI":"10.1159\/000489873","type":"journal-article","created":{"date-parts":[[2018,6,18]],"date-time":"2018-06-18T21:01:25Z","timestamp":1529355685000},"page":"393-396","source":"Crossref","is-referenced-by-count":4,"title":["Validation of a New Low-Cost, Methanol-Based Fixative for Cervical Cytology and Human Papillomavirus Detection"],"prefix":"10.1159","volume":"62","author":[{"given":"Katia\u00a0Ramos\u00a0Moreira","family":"Leite","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna","family":"Naum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavio","family":"Canavez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Canavez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruan","family":"Pimenta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Reis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz\u00a0Heraldo","family":"Camara-Lopes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2018,6,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2009.1569"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20599"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2008.12.012"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159\/000435801"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20656"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(200001)22:1<52::AID-DC14>3.0.CO;2-#"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(09)70002-2"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.20203"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.21521"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.3310\/hta8200"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007\/s10552-015-0678-1"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.j504"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.11.1500"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/489873","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/489873","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:33:37Z","timestamp":1745426017000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000489873"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":13,"journal-issue":{"issue":"5-6","published-online":{"date-parts":[[2018,12,4]]},"published-print":{"date-parts":[[2018,12,7]]}},"URL":"https:\/\/doi.org\/10.1159\/000489873","archive":["Portico"],"relation":{},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"value":"0001-5547","type":"print"},{"value":"1938-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}