{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T08:58:09Z","timestamp":1790499489187,"version":"4.1.0"},"reference-count":0,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Innate Immun"],"published-print":{"date-parts":[[2018]]},"abstract":"In this study, we analyzed interferon (IFN)-\u03b3-producing cells and M1\/M2 macrophage polarization in <i>Legionella pneumophila<\/i> pneumonia following anti-Gr-1 antibody treatment. Anti-Gr-1 treatment induced an M1-to-M2 shift of macrophage subtypes in the lungs and weakly in the peripheral blood, which was associated with increased mortality in legionella-infected mice. CD8<sup>+<\/sup> T lymphocytes and natural killer cells were the dominant sources of IFN-\u03b3 in the acute phase, and anti-Gr-1 treatment reduced the number of IFN-\u03b3-producing CD8<sup>+<\/sup> T lymphocytes. In the CD3-gated population, most Gr-1-positive cells were CD8<sup>+<\/sup> T lymphocytes in the lungs and lymph nodes (LNs) of infected mice. Additionally, the number of IFN-\u03b3-producing Gr-1<sup>+<\/sup> CD8<sup>+<\/sup> T lymphocytes in the lungs and LNs increased 2 and 4 days after <i>L.\u00a0pneumophila<\/i> infection, with anti-Gr-1 treatment attenuating these populations. Antibody staining revealed that Gr-1<sup>+<\/sup> CD8<sup>+<\/sup> T lymphocytes were Ly6C-positive cells rather than Ly6G, a phenotype regarded as memory type cells. Furthermore, the adoptive transfer of Gr-1<sup>+<\/sup> CD8<sup>+<\/sup> T lymphocytes induced increases in IFN-\u03b3, M1 shifting and reduced bacterial number in the <i>Legionella<\/i> pneumonia model. These data identified Ly6C<sup>+<\/sup> CD8<sup>+<\/sup> T lymphocytes as a source of IFN-\u03b3 in innate immunity and partially associated with reduced IFN-\u03b3 production, M2 polarization, and high mortality in anti-Gr-1 antibody-treated mice with <i>L. pneumophila<\/i> pneumonia.<\/jats:p>","DOI":"10.1159\/000490585","type":"journal-article","created":{"date-parts":[[2018,7,18]],"date-time":"2018-07-18T21:04:25Z","timestamp":1531947865000},"page":"328-338","source":"Crossref","is-referenced-by-count":17,"title":["Potential Role of Gr-1<sup>+<\/sup> CD8<sup>+<\/sup> T Lymphocytes as a Source of Interferon-\u03b3 and M1\/M2 Polarization during the Acute Phase of Murine <b><i>Legionella pneumophila<\/i><\/b> Pneumonia"],"prefix":"10.1159","volume":"10","author":[{"given":"Yu","family":"Kusaka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiaki","family":"Kajiwara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sho","family":"Shimada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikazu","family":"Ishii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasunari","family":"Miyazaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naohiko","family":"Inase","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodore\u00a0J.","family":"Standiford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Tateda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2018,7,18]]},"container-title":["Journal of Innate Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/490585","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/490585","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:40:26Z","timestamp":1745426426000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000490585"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,9,26]]},"published-print":{"date-parts":[[2018,9,27]]}},"URL":"https:\/\/doi.org\/10.1159\/000490585","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.6834461","asserted-by":"subject"}]},"ISSN":["1662-811X","1662-8128"],"issn-type":[{"value":"1662-811X","type":"print"},{"value":"1662-8128","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}