{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T15:50:26Z","timestamp":1769097026517,"version":"3.49.0"},"reference-count":0,"publisher":"S. Karger AG","issue":"5-6","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathobiology"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; This study was designed to evaluate the prevalence of &lt;i&gt;CTNNB1&lt;\/i&gt; (\u03b2-catenin) mutations in cases of papillary thyroid carcinoma with desmoid-type fibromatosis (PTC-DTF) expressing aberrant nuclear and cytoplasmic immunoreactivity for \u03b2-catenin. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Eight cases of PTC-DTF were available for this study. Immunohistochemistry for \u03b2-catenin and BRAF&lt;sup&gt;V600E&lt;\/sup&gt; was performed. &lt;i&gt;CTNNB1&lt;\/i&gt; and &lt;i&gt;BRAFV600E&lt;\/i&gt; mutations were also evaluated by direct sequencing. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; For \u03b2-catenin, although we could demonstrate aberrant nuclear and cytoplasmic immunoreactivity in DTF components in all cases, suggesting activated Wnt signaling, direct sequencing revealed a missense mutation, c.121A&amp;#x3e;G (p.T41A), in exon 3 in only one case, and no mutations in exons 3, 4, and 5 in the other cases. In the BRAF&lt;sup&gt;V600E&lt;\/sup&gt; analyses, immunohistochemistry revealed positive staining in the carcinoma cells but not DTF components of all cases. These findings were subsequently validated by direct sequencing. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; This study suggests the significance of the &lt;i&gt;BRAFV600E&lt;\/i&gt; mutation and activation of Wnt signaling pathway in the carcinoma cells and DTF components, respectively. We believe that the &lt;i&gt;CTNNB1&lt;\/i&gt; mutations are not the major factor behind \u03b2-catenin translocation indicating Wnt pathway activation. Further study is required to evaluate whether molecular abnormalities other than the &lt;i&gt;CTNNB1&lt;\/i&gt; mutation cause activation of Wnt signaling in DTF components of PTC-DTF.<\/jats:p>","DOI":"10.1159\/000492117","type":"journal-article","created":{"date-parts":[[2018,9,17]],"date-time":"2018-09-17T21:01:20Z","timestamp":1537218080000},"page":"300-303","source":"Crossref","is-referenced-by-count":15,"title":["Immunohistochemical and Molecular Analyses Focusing on Mesenchymal Cells in Papillary Thyroid Carcinoma with Desmoid-Type Fibromatosis"],"prefix":"10.1159","volume":"85","author":[{"given":"Nami","family":"Takada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhanna","family":"Mussazhanova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuyoshi","family":"Hirokawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Nakashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Miyauchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2018,9,17]]},"container-title":["Pathobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/492117","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/492117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:51:55Z","timestamp":1745427115000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000492117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":0,"journal-issue":{"issue":"5-6","published-online":{"date-parts":[[2018,11,15]]},"published-print":{"date-parts":[[2018,11,27]]}},"URL":"https:\/\/doi.org\/10.1159\/000492117","archive":["Portico"],"relation":{},"ISSN":["1015-2008","1423-0291"],"issn-type":[{"value":"1015-2008","type":"print"},{"value":"1423-0291","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}