{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:55:53Z","timestamp":1761238553545,"version":"3.40.4"},"reference-count":0,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2018,9,11]],"date-time":"2018-09-11T00:00:00Z","timestamp":1536624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2018,9,11]],"date-time":"2018-09-11T00:00:00Z","timestamp":1536624000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Innate Immun"],"published-print":{"date-parts":[[2019]]},"abstract":"<jats:p>It has emerged that neutrophils can play important roles in the host response following infection with helminth parasites. Mice infected with the tapeworm, &lt;i&gt;Hymenolepis diminuta&lt;\/i&gt;, are protected from some inflammatory conditions, accompanied by reduced neutrophil tissue infiltration. Thus, the ability of a phosphate-buffered saline-soluble extract of the worm (&lt;i&gt;H. diminuta&lt;\/i&gt; extract [HdE]) was tested for (1) its ability to activate murine neutrophils (Ca&lt;sup&gt;2+&lt;\/sup&gt; mobilization, reactive oxygen species (ROS) and cytokine production); and (2) affect neutrophil chemotaxis in vitro to the penta-peptide, WKYMVm, the chemokine, KC, and leukotriene B&lt;sub&gt;4&lt;\/sub&gt;. HdE was not cytotoxic to neutrophils, elicited a Ca&lt;sup&gt;2+&lt;\/sup&gt; response and ROS, but not, cytokine (KC, interleukin-10, tumour necrosis factor-\u03b1) generation. HdE is not a chemotactic stimulus for murine neutrophils. However, a heat- and trypsin-sensitive, acid-insensitive proteoglycan (sensitive to sodium metaperiodate) in the HdE significantly reduced neutrophil chemotaxis towards WKYMVm or KC, but not LTB&lt;sub&gt;4&lt;\/sub&gt;. The latter suggested that the HdE interfered with p38 mitogen-activated protein kinase signalling, which is important in WKYMVm chemotaxis. Corroborating this, immunoblotting revealed reduced phosphorylated p38, and the downstream signal heat-shock protein-27, in protein extracts from HdE + WkYMVm treated cells compared to those exposed to the penta-peptide only. We speculate that HdE can be used to modify the outcome of neutrophilic disease and that purification of the bioactive proteoglycan(s) from the extract could be used as a template to design immunomodulatory drugs targeting neutrophils.<\/jats:p>","DOI":"10.1159\/000492303","type":"journal-article","created":{"date-parts":[[2018,9,11]],"date-time":"2018-09-11T21:04:26Z","timestamp":1536699866000},"page":"136-149","source":"Crossref","is-referenced-by-count":8,"title":["A Trypsin-Sensitive Proteoglycan from the Tapeworm &lt;b&gt;&lt;i&gt;Hymenolepis diminuta&lt;\/i&gt;&lt;\/b&gt; Inhibits Murine Neutrophil Chemotaxis in vitro by Suppressing p38 MAP Kinase Activation"],"prefix":"10.1159","volume":"11","author":[{"given":"Nicholas","family":"Graves","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivek\u00a0P.","family":"Venu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan\u00a0G.","family":"Yipp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bj\u00f6rn","family":"Petri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon","family":"Hirota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Gilleard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek\u00a0M.","family":"McKay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2018,9,11]]},"container-title":["Journal of Innate Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/492303","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/492303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:53:50Z","timestamp":1745427230000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000492303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,11]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,3,6]]},"published-print":{"date-parts":[[2019,3,8]]}},"URL":"https:\/\/doi.org\/10.1159\/000492303","archive":["Portico"],"relation":{},"ISSN":["1662-811X","1662-8128"],"issn-type":[{"type":"print","value":"1662-811X"},{"type":"electronic","value":"1662-8128"}],"subject":[],"published":{"date-parts":[[2018,9,11]]}}}