{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T08:14:05Z","timestamp":1790928845665,"version":"4.1.0"},"reference-count":14,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T00:00:00Z","timestamp":1540252800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T00:00:00Z","timestamp":1540252800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2019]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Objectives:&lt;\/i&gt;&lt;\/b&gt; To validate the ecological modification of the Papanicolaou stain (Eco-Pap) for the diagnosis of cervical cancer. &lt;b&gt;&lt;i&gt;Study Design:&lt;\/i&gt;&lt;\/b&gt; A prospective study was performed at the Mother and Child \u201cSan Bartolom\u00e9\u201d Hospital, Lima, Peru. Reagent handling strategies were divided into three phases: we used (1) Harris progressive hematoxylin (for nuclear staining), (2) a polychromatic solution (a mix of EA-36\/Orange G-6 to suppress the use of several alcohol baths), and (3) direct mounting (with Entellan\u00ae solution). The cellular details were analyzed by the staining quality index, an external quality control, and the Bethesda System 2014. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; We evaluated 72,901 cervical smears stained with the Eco-Pap. The validation of the Eco-Pap against the conventional Pap stain was optimal (\u03ba = 0.89; 95% CI: 0.87\u20130.92), showing a sensibility and specificity of 88.3% (95% CI: 85.1\u201390.0) and 98.7% (95% CI: 98\u201399.2), respectively. The Eco-Pap dramatically reduced the environmental pollution caused by 72 L of xylene, hydrochloric acid, and ammonia (6 L each) and mercury oxide. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; The Eco-Pap is an innovative and efficient staining method reducing the use of toxic reagents with carcinogenic potential during cervical cancer screening by exfoliative cytology.<\/jats:p>","DOI":"10.1159\/000493113","type":"journal-article","created":{"date-parts":[[2018,10,23]],"date-time":"2018-10-23T21:05:16Z","timestamp":1540328716000},"page":"35-43","source":"Crossref","is-referenced-by-count":10,"title":["Eco-Pap: The Ecological Modification of the Papanicolaou Stain for Sustainable Cervical Cancer Diagnosis"],"prefix":"10.1159","volume":"63","author":[{"given":"Jeel","family":"Moya-Salazar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor","family":"Rojas-Zumaran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2018,10,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(16)40621-6"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.5306\/wjco.v6.i6.281"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.patol.2015.12.001"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1136\/ewjm.175.4.231"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1159\/000326034"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1159\/000444117"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.101129"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1093\/labmed\/8.4.16"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.5696\/2156-9614-6-11.58"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1159\/000441515"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-407-72901-8.50028-7"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20360"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.ogc.2008.09.006"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.4103\/0973-029X.64299"}],"container-title":["Acta Cytologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/493113","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/493113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:48:31Z","timestamp":1745426911000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000493113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,23]]},"references-count":14,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,2,20]]},"published-print":{"date-parts":[[2019,2,26]]}},"URL":"https:\/\/doi.org\/10.1159\/000493113","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.7240925","asserted-by":"subject"}]},"ISSN":["0001-5547","1938-2650"],"issn-type":[{"value":"0001-5547","type":"print"},{"value":"1938-2650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,23]]}}}