{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T01:32:01Z","timestamp":1775007121002,"version":"3.50.1"},"reference-count":0,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Med Princ Pract"],"published-print":{"date-parts":[[2018]]},"abstract":"<b><i>Objective:<\/i><\/b> <i>Candida albicans<\/i> and <i>Candida dubliniensis<\/i> are germ tube-positive pathogenic yeast species. Accurate identification of these two species is warranted since <i>C. albicans<\/i> is a highly pathogenic species while <i>C. dubliniensis<\/i> exhibits increased adherence to buccal epithelial cells, reduced susceptibility to azoles and resistance to flucytosine. We have developed a duplex real-time PCR assay for rapid detection and differentiation between clinical <i>C. albicans<\/i> and <i>C. dubliniensis<\/i> isolates. <b><i>Materials and Methods:<\/i><\/b> A duplex real-time PCR assay was developed by using two species-specific primer pairs and SYBR Green dye to differentiate <i>C. albicans<\/i> and <i>C. dubliniensis<\/i> isolates via melting curve analysis of real-time PCR amplicons. Amplification products were also analyzed by agarose gel electrophoresis to confirm real-time PCR results. <b><i>Results:<\/i><\/b> Melting temperatures (<i>T<\/i><sub><i>m<\/i><\/sub>) for reference strains of <i>C. albicans<\/i> and <i>C. dubliniensis<\/i> were 86.55 and 82.75\u00b0C, respectively. No amplicon was obtained with DNA from reference strains of 8 other common <i>Candida<\/i> spp. When real-time PCR was applied on 226 clinical isolates previously identified by the Vitek 2 system and\/or PCR sequencing of rDNA, <i>T<\/i><sub><i>m<\/i><\/sub> values for <i>C. albicans<\/i> (<i>n<\/i> = 113) and <i>C. dubliniensis<\/i> (<i>n<\/i> = 98) were 86.68 \u00b1 0.529 and 82.616 \u00b1 0.535\u00b0C, respectively. The results were confirmed by agarose gel electrophoresis. No amplicon was obtained from 15 isolates belonging to 9 other <i>Candida<\/i> spp. <b><i>Conclusions:<\/i><\/b> The real-time PCR assay described here does not require prior identification of clinical yeast isolates as <i>C. albicans<\/i>\/<i>C. dubliniensis<\/i> by germ tube formation and accurately reports results within 2 h. Detection of amplicons by agarose gel electrophoresis is also suitable for resource-poor settings devoid of real-time PCR facilities.<\/jats:p>","DOI":"10.1159\/000493426","type":"journal-article","created":{"date-parts":[[2018,9,3]],"date-time":"2018-09-03T21:01:26Z","timestamp":1536008486000},"page":"543-548","source":"Crossref","is-referenced-by-count":23,"title":["Rapid and Accurate Identification of <b><i>Candida albicans<\/i><\/b> and <b><i>Candida dubliniensis<\/i><\/b> by Real-Time PCR and Melting Curve Analysis"],"prefix":"10.1159","volume":"27","author":[{"given":"Mohammad","family":"Asadzadeh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suhail","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noura","family":"Al-Sweih","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziauddin","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2018,9,3]]},"container-title":["Medical Principles and Practice"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/493426","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/493426","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:51:45Z","timestamp":1745427105000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000493426"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2019,3,1]]},"published-print":{"date-parts":[[2019,3,8]]}},"URL":"https:\/\/doi.org\/10.1159\/000493426","archive":["Portico"],"relation":{},"ISSN":["1011-7571","1423-0151"],"issn-type":[{"value":"1011-7571","type":"print"},{"value":"1423-0151","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}