{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T08:30:58Z","timestamp":1762504258684,"version":"3.40.5"},"reference-count":69,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fetal Diagn Ther"],"published-print":{"date-parts":[[2019]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Introduction:&lt;\/i&gt;&lt;\/b&gt; Significant limitations with existing treatments for major haemoglobinopathies motivate the development of effective intrauterine therapy. We assessed the feasibility of fetoscopic and ultrasound-guided intrauterine haemopoietic cell transplantation (IUHCT) in macaque fetuses in early gestation when haemopoietic and immunological ontogeny is anticipated to enable long-term donor cell engraftment. &lt;b&gt;&lt;i&gt;Material and Methods:&lt;\/i&gt;&lt;\/b&gt; Fluorescent-labelled bone marrow-derived mononuclear cells from 10 pregnant &lt;i&gt;Macaca fascicularis&lt;\/i&gt; were injected into their fetuses at E71\u2013114 (18.9\u2013170.0E+6 cells\/fetus) by fetoscopic intravenous (&lt;i&gt;n&lt;\/i&gt; = 7) or ultrasound (US)-guided intracardiac injections, with sacrifice at 24 h to examine donor-cell distribution. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Operating times ranged from 35 to 118 min. Chorionic membrane tenting and intrachorionic haemorrhage were observed only with fetoscopy (&lt;i&gt;n&lt;\/i&gt; = 2). Labelled cells were stereoscopically visualised in lung, spleen, liver, and placenta. Donor-cell chimerism was highest in liver, spleen, and heart by flow cytometry, placenta by unique polymorphism qPCR, and was undetected in blood. Chimerism was 2\u20133 log-fold lower in individual organs by qPCR than by flow cytometry. &lt;b&gt;&lt;i&gt;Discussion:&lt;\/i&gt;&lt;\/b&gt; Both fetoscopic and US-guided IUHCT were technically feasible, but fetoscopy caused more intraoperative complications in our pilot series. The discrepancy in chimerism detection predicts the challenges in long-term surveillance of donor-cell chimerism. Further studies of long-term outcomes in the non-human primate are valuable for the development of clinical protocols for IUHCT.<\/jats:p>","DOI":"10.1159\/000493791","type":"journal-article","created":{"date-parts":[[2019,1,18]],"date-time":"2019-01-18T22:01:19Z","timestamp":1547848879000},"page":"175-186","source":"Crossref","is-referenced-by-count":3,"title":["Fetoscopic versus Ultrasound-Guided Intravascular Delivery of Maternal Bone Marrow Cells in Fetal Macaques: A Technical Model for Intrauterine Haemopoietic Cell Transplantation"],"prefix":"10.1159","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0068-6957","authenticated-orcid":false,"given":"Citra\u00a0N.Z.","family":"Mattar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Wan","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuryanti","family":"Johana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arijit","family":"Biswas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lay-Geok","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahesh","family":"Choolani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Bakkour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerry\u00a0K.Y.","family":"Chan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan\u00a0W.","family":"Flake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2019,1,18]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2012.06.006"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2012.06.005"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2014.00251"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2014.00278"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4536"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.3109\/03630269.2014.984071"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2014-308112"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61229-X"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001484"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08742.x"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.2011.585153"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24231"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1185\/03007995.2015.1110128"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-12-674606"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.25911"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740-8709.2012.00454.x"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2012.06.004"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genom.5.061903.175935"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2015.08.006"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1089\/15258160260194767"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-11-537571"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.3791\/2303"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2009.11.005"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1159\/000081367"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1159\/000203399"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2008.11.034"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-10-463174"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1172\/JCI44907"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2003.12.008"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-06-723981"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2016.05.009"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-02-550327"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2004.12.002"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.4161\/chim.24914"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1038\/nature22795"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2014.05.025"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1159\/000346211"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.4161\/chim.1.2.13147"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-12-128116"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2018.03.006"},{"key":"ref41","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-016-2620-3"},{"key":"ref42","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2013.08.008"},{"key":"ref43","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2017.26"}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