{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T11:12:14Z","timestamp":1769425934121,"version":"3.49.0"},"reference-count":28,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T00:00:00Z","timestamp":1542585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T00:00:00Z","timestamp":1542585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"published-print":{"date-parts":[[2019]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt; Limited supply and complicated manufacturing procedure of animal-derived surfactants make the development of synthetic surfactants warranted. The synthesis of surfactant protein (SP)-B and SP-C is complicated and several analogues have been developed. Mini-BLeu is an analogue that corresponds to the first and last helix of SP-B joined by a loop and linked by 2 disulphide bridges. SP-C33Leu is an SP-C analogue that can be cost-efficiently produced, but no such analogue has yet been described for SP-B. &lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; To design short SP-B analogues which lack disulphide bridges, are easy to produce and are efficacious in a preterm rabbit fetus model of neonatal RDS. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Synthetic surfactants were prepared by adding 2 or 8% (w\/w) of synthetic variants of Mini-B27, similar to Mini-BLeu but with a short loop, or different peptides covering helix 1 of SP-B to 2% (w\/w) of SP-C33Leu in 80 mg\/mL of 1,2-dipalmitoyl-&lt;i&gt;sn&lt;\/i&gt;-glycero-3-phosphocholine\/egg yolk phosphatidylcholine\/1-palmitoyl-2-oleoyl-&lt;i&gt;sn&lt;\/i&gt;-glycero-3-phosphoglycerol, 50: 40: 10 (by weight). Premature newborn rabbit fetuses were treated with 200 mg\/kg of the surfactant preparations and ventilated with defined pressures for 30 min without positive end-expiratory pressure. Tidal volumes were registered during the experiments and lung gas volumes were measured at the end of the ventilation period. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Synthetic surfactant containing the Mini-B27 analogue with 2 disulphides gives similar lung gas volumes as treatment with an animal-derived surfactant preparation, but all other SP-B analogues gave lower lung gas volumes. All synthetic surfactants studied gave no significant differences in compliances except the surfactant containing the Mini-B27 analogue without cysteines that performed somewhat better at 30 min. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; The helix-loop-helix SP-B analogues tested in this study require the presence of 2 disulphide bridges for optimal activity in a rabbit RDS model.<\/jats:p>","DOI":"10.1159\/000494100","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T22:01:45Z","timestamp":1542664905000},"page":"134-141","source":"Crossref","is-referenced-by-count":3,"title":["Disulphide Bridges in Surfactant Protein B Analogues Affect Their Activity in Synthetic Surfactant Preparations"],"prefix":"10.1159","volume":"115","author":[{"given":"Oihana","family":"Basabe-Burgos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Johansson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tore","family":"Curstedt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2018,11,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1159\/000349942"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(01)00308-7"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2014.09.002"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1159\/000442874"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.162.4.9908104"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1987.tb13414.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00865.2007"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1159\/000276980"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)01692-0"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1021\/bi00242a015"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1021\/bi0485575"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.56.11.871"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3011.2005.00300.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1021\/bi7011756"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039392"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1021\/bi00185a042"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560071206"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00153.2003"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038\/pr.2016.231"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1159\/000356065"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2017-312722"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh371"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1002\/bip.20853"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047631"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(90)90155-F"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1021\/bi00699a001"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.12688\/gatesopenres.12799.1"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008672"}],"container-title":["Neonatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/494100","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/494100","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:00:43Z","timestamp":1745427643000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000494100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,19]]},"references-count":28,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,2,28]]},"published-print":{"date-parts":[[2019,3,7]]}},"URL":"https:\/\/doi.org\/10.1159\/000494100","archive":["Portico"],"relation":{},"ISSN":["1661-7800","1661-7819"],"issn-type":[{"value":"1661-7800","type":"print"},{"value":"1661-7819","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,19]]}}}