{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:21:18Z","timestamp":1790882478042,"version":"4.1.0"},"reference-count":0,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microb Physiol"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>The aim of this work was the genetic characterization of cefotaxime-resistant enterobacteria from animals (53 samples), the surface water of rivers (17 samples), and wastewater treatment plants (43 samples) in Tunisia. A total of 48 (42.4%) cefotaxime-resistant isolates were recovered. An extended spectrum beta-lactamase (ESBL) phenotype with a positive double-disk synergy test (DDST) was exhibited by 34 (70.8%) and 14 (29.1%) isolates from water and animal origins, respectively. Isolates from water were identified as: &lt;i&gt;Escherichia coli&lt;\/i&gt; (&lt;i&gt;n&lt;\/i&gt; = 17), &lt;i&gt;Hafnia&lt;\/i&gt; spp&lt;i&gt;.&lt;\/i&gt; (&lt;i&gt;n&lt;\/i&gt; = 13), &lt;i&gt;Citrobacter&lt;\/i&gt; spp&lt;i&gt;.&lt;\/i&gt; (&lt;i&gt;n&lt;\/i&gt; = 1), &lt;i&gt;Enterobacter cloacae&lt;\/i&gt; (&lt;i&gt;n&lt;\/i&gt; = 1), &lt;i&gt;Klebsiella pneumoniae&lt;\/i&gt;  (&lt;i&gt;n&lt;\/i&gt; = 1), and &lt;i&gt;K. oxytoca&lt;\/i&gt; (&lt;i&gt;n&lt;\/i&gt; = 1). Animal isolates were identified as: &lt;i&gt;E. coli&lt;\/i&gt; (&lt;i&gt;n&lt;\/i&gt; = 11), &lt;i&gt;E. cloacae&lt;\/i&gt; (&lt;i&gt;n&lt;\/i&gt; = 1), &lt;i&gt;Hafnia&lt;\/i&gt; spp&lt;i&gt;.&lt;\/i&gt; (&lt;i&gt;n&lt;\/i&gt; = 1), and &lt;i&gt;K. pneumoniae&lt;\/i&gt; (&lt;i&gt;n&lt;\/i&gt; = 1). PCR investigation of &lt;i&gt;bla&lt;\/i&gt;&lt;sub&gt;CTX-M&lt;\/sub&gt;, &lt;i&gt;bla&lt;\/i&gt;&lt;sub&gt;TEM&lt;\/sub&gt;, and &lt;i&gt;bla&lt;\/i&gt;&lt;sub&gt;SHV&lt;\/sub&gt; genes showed that amongst the 48 isolates with a positive DDST, 41 (87.5%) carried the &lt;i&gt;bla&lt;\/i&gt;&lt;sub&gt;CTX-M&lt;\/sub&gt; gene, 1 isolate harbored the &lt;i&gt;bla&lt;\/i&gt;&lt;sub&gt;SHV&lt;\/sub&gt; gene, and 1 isolate coharbored &lt;i&gt;bla&lt;\/i&gt;&lt;sub&gt;CTX-M&lt;\/sub&gt; with &lt;i&gt;bla&lt;\/i&gt;&lt;sub&gt;SHV&lt;\/sub&gt; genes. The class 1 and 2 integrons were detected in 27 (56.2%) and 1 (2%) isolates, respectively. Our study showed a significant occurrence of ESBL-producing enterobacteria in animals and aquatic environments with a predominance of &lt;i&gt;bla&lt;\/i&gt;&lt;sub&gt;CTX-M&lt;\/sub&gt; genes.<\/jats:p>","DOI":"10.1159\/000495409","type":"journal-article","created":{"date-parts":[[2019,1,4]],"date-time":"2019-01-04T22:01:21Z","timestamp":1546639281000},"page":"201-206","source":"Crossref","is-referenced-by-count":3,"title":["Multidrug Resistance and the Predominance of &lt;b&gt;&lt;i&gt;bla&lt;\/i&gt;&lt;\/b&gt;&lt;sub&gt;CTX-M&lt;\/sub&gt; in&lt;sub&gt;&lt;\/sub&gt; Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae of Animal and Water Origin"],"prefix":"10.1159","volume":"28","author":[{"given":"Bilel","family":"Hassen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senda","family":"Sghaier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed\u00a0Salah","family":"Abbassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed\u00a0Amine","family":"Ferjani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meriam","family":"Ben\u00a0Said","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdennaceur","family":"Hassen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salah","family":"Hammami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2019,1,4]]},"container-title":["Microbial Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/495409","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/495409","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:13:53Z","timestamp":1745428433000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000495409"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2019,1,18]]},"published-print":{"date-parts":[[2019,1,24]]}},"URL":"https:\/\/doi.org\/10.1159\/000495409","archive":["Portico"],"relation":{},"ISSN":["2673-1665","2673-1673"],"issn-type":[{"value":"2673-1665","type":"print"},{"value":"2673-1673","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}