{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T06:13:38Z","timestamp":1773296018395,"version":"3.50.1"},"reference-count":24,"publisher":"S. Karger AG","issue":"1","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/karger.com\/pages\/terms-and-conditions"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2019]]},"abstract":"<jats:p>Background: The aim of this study was to investigate the role of Notch-1 signaling through Notch-1 ligands on bronchial epithelial cells (BECs) in regulating the development of T helper 2 (Th2) lymphocytes after RSV infection. Methods: Firstly, we analyzed the expression of cytokines and Notch-1 ligands in BECs by using real-time PCR. Then, RSV-infected BECs were co-cultured with CD4+ T cells in a transwell chamber for 48 h, and differentiation of T cells in the lower chamber was determined using flow cytometry and real-time PCR. JAG1 siRNA was then used to determine the effects of Jagged\/Notch-1 signaling on the differentiation of Th2. An RSV-infected mouse model was also used to analyze the secretion of Th differentiation-associated cytokines in serum and lung tissues using ELISA, the histopathological changes using HE staining, and the expression of JAG1 and JAG2 in BECs. Results: The results showed that RSV promoted the expression of Th2-type cytokines and Jagged-1 and inhibited the expression of Jagged-2 in normal BECs. RSV-infected BECs induced Th2 differentiation. In addition, JAG1 downregulation inhibited the differentiation of Th2 and promoted differentiation of Th1. In the RSV-infected mouse model, the RSV titer, inflammation decreased with time. IL-4 and IL-17 increased on day 28 and 60, while IFN\u03b3 increased on day 7 and 28. Moreover, the expression of Jagged-1 increased and that of Jagged-2 decreased in BECs, which was consistent with IL-4 production in lung tissues. Conclusion: Our data showed that BECs had the potential to promote the differentiation of Th2 lymphocytes through Jagged-1\/Notch-1 signaling.<\/jats:p>","DOI":"10.1159\/000495581","type":"journal-article","created":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T21:55:32Z","timestamp":1554328532000},"page":"43-52","source":"Crossref","is-referenced-by-count":23,"title":["Bronchial Epithelial Cells Promote the Differentiation of Th2 Lymphocytes in Airway Microenvironment through Jagged\/Notch-1 Signaling after RSV Infection"],"prefix":"10.1159","volume":"179","author":[{"given":"Ling","family":"Qin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke-zi","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng-ping","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-jun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-rong","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2019,4,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/apa.12662"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0087162"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2007.10.020"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.3390\/ijms140918024"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-012-2107-9"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000581"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1159\/000436966"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1159\/000485895"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159\/000485890"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2009.06.002"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2008-0449OC"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.01.004"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00169"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2617"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.21037\/jtd.2016.06.37"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01754"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01177"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.12.1950"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038\/ni1247"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2009-0418OC"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201403-0505OC"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2017.0078"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-013-1116-3"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00451-9"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/karger.com\/iaa\/article-pdf\/179\/1\/43\/3907609\/000495581.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/karger.com\/iaa\/article-pdf\/179\/1\/43\/3907609\/000495581.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T17:15:54Z","timestamp":1745428554000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000495581"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":24,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,5,3]]},"published-print":{"date-parts":[[2019,1,1]]}},"URL":"https:\/\/doi.org\/10.1159\/000495581","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]}}}