{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T04:51:05Z","timestamp":1773204665644,"version":"3.50.1"},"reference-count":64,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuroendocrinology"],"published-print":{"date-parts":[[2019]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background\/Aims:&lt;\/i&gt;&lt;\/b&gt; Nesfatin-1, processed from nucleobindin-2 (NUCB2), is a potent anorexigenic peptide being expressed in rodent hypothalamic nuclei and involved in the regulation of feeding behavior and body weight in animals. The present study aimed to investigate NUCB2\/nesfatin-1 protein expression in the human hypothalamus as well as its correlation with body weight. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Sections of hypothalamus and adjacent cholinergic basal forebrain nuclei, including the nucleus basalis of Meynert (NBM) and the diagonal band of Broca (DBB), from 25 autopsy cases (17 males, 8 females; 8 lean, 9 overweight, 8 obese) were examined using immunohistochemistry and double immunofluorescence labeling. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Prominent NUCB2\/nesfatin-1 immunoexpression was detected in supraoptic, paraventricular, and infundibular nuclei, lateral hypothalamic area (LHA)\/perifornical region, and NBM\/DBB. NUCB2\/nesfatin-1 was found to extensively colocalize with (a) oxytocin and vasopressin in paraventricular and supraoptic nuclei, (b) melanin-concentrating hormone in the LHA, and (c) cocaine- and amphetamine-regulated transcript in infundibular and paraventricular nuclei and LHA. Interestingly, in the LHA, NUCB2\/nesfatin-1 protein expression was significantly decreased in obese, compared with lean (&lt;i&gt;p&lt;\/i&gt; &amp;#x3c; 0.01) and overweight (&lt;i&gt;p&lt;\/i&gt; &amp;#x3c; 0.05) subjects. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; The findings of the present study are suggestive of a potential role for NUCB2\/nesfatin-1 as an integral regulator of food intake and energy homeostasis in the human hypothalamus. In the LHA, an appetite- and reward-related brain area, reduced NUCB2\/nesfatin-1 immunoexpression may contribute to dysregulation of homeostatic and\/or hedonic feeding behavior and obesity. NUCB2\/nesfatin-1 localization in NBM\/DBB might imply its participation in the neuronal circuitry controlling cognitive influences on food intake and give impetus towards unraveling additional biological actions of NUCB2\/nesfatin-1 in human neuronal networks.<\/jats:p>","DOI":"10.1159\/000496731","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T22:05:44Z","timestamp":1547071544000},"page":"190-200","source":"Crossref","is-referenced-by-count":25,"title":["Nucleobindin-2\/Nesfatin-1 in the Human Hypothalamus Is Reduced in Obese Subjects and Colocalizes with Oxytocin, Vasopressin, Melanin-Concentrating Hormone, and Cocaine- and Amphetamine-Regulated Transcript"],"prefix":"10.1159","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9609-6710","authenticated-orcid":false,"given":"Aristea","family":"Psilopanagioti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia","family":"Nikou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Papadaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2019,1,9]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2015.103"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05162"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-16-0361"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-0701"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2008.07.054"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2009.01.064"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2011.04.031"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.2174\/138161281939131127124814"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.12717\/DR.2014.18.4.301"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-0747"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-013-1087-8"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1210\/en.2009-1358"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-011-1268-5"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.02.104"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1210\/en.2011-2032"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2007.09.005"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2007.10.008"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-1782"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1210\/en.2009-0578"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2009.09.002"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-0598"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1097\/WNR.0b013e32833f7b96"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2010.09.027"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2012.03.015"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2012.04.003"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.12366"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1097\/WNR.0000000000000293"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.12973"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2016.10.010"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-1276"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b12-00505"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1677\/JME-10-0059"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00415-9"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-007-9519-2"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod64.5.1466"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2005.04.032"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.08.017"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(85)90160-5"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2010.05.002"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2008.05.035"},{"key":"ref41","doi-asserted-by":"publisher","DOI":"10.1016\/j.npep.2011.12.002"},{"key":"ref42","doi-asserted-by":"publisher","DOI":"10.1210\/en.2015-2082"},{"key":"ref43","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2017.03.007"},{"key":"ref44","doi-asserted-by":"publisher","DOI":"10.2337\/db13-0663"},{"key":"ref45","doi-asserted-by":"publisher","DOI":"10.1002\/oby.21069"},{"key":"ref46","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(91)90251-I"},{"key":"ref47","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2012.02304.x"},{"key":"ref48","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00969-8"},{"key":"ref49","doi-asserted-by":"publisher","DOI":"10.1210\/mend-4-4-632"},{"key":"ref50","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2017.10.015"},{"key":"ref51","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2009.11.024"},{"key":"ref52","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1714-04.2005"},{"key":"ref53","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4220"},{"key":"ref54","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9384(02)00756-4"},{"key":"ref55","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2005.10.007"},{"key":"ref56","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-23-09273.2001"},{"key":"ref57","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2009.06765.x"},{"key":"ref58","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(82)90365-1"},{"key":"ref59","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam7100"},{"key":"ref60","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2016.00634"},{"key":"ref61","doi-asserted-by":"publisher","DOI":"10.1101\/lm.305306"},{"key":"ref62","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2008.04.012"},{"key":"ref63","doi-asserted-by":"publisher","DOI":"10.1038\/nature19789"},{"key":"ref64","doi-asserted-by":"publisher","DOI":"10.1007\/s12603-015-0595-8"}],"container-title":["Neuroendocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/496731","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/496731","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:11:55Z","timestamp":1745424715000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000496731"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":64,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2019,4,26]]},"published-print":{"date-parts":[[2019,4,30]]}},"URL":"https:\/\/doi.org\/10.1159\/000496731","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.7803635","asserted-by":"subject"}]},"ISSN":["0028-3835","1423-0194"],"issn-type":[{"value":"0028-3835","type":"print"},{"value":"1423-0194","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]}}}