{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T16:30:31Z","timestamp":1772641831850,"version":"3.50.1"},"reference-count":23,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Haematol"],"published-print":{"date-parts":[[2019]]},"abstract":"<i>BCR-ABL1<\/i>-negative myeloproliferative disorders and chronic myeloid leukaemia are haematologic malignancies characterised by single and mutually exclusive genetic alterations. Nevertheless, several patients co-expressing the <i>JAK2<\/i><sup>V617F<\/sup> mutation and the <i>BCR-ABL1<\/i> transcript have been described in the literature. We report the case of a 61-year-old male who presented with an essential thrombocythaemia phenotype and had a subsequent diagnosis of chronic phase chronic myeloid leukaemia. Colony-forming assays demonstrated the coexistence of 2 different haematopoietic clones: one was positive for the <i>JAK2<\/i><sup>V617F<\/sup> mutation and the other co-expressed both <i>JAK2<\/i><sup>V617F<\/sup> and the <i>BCR-ABL1<\/i> fusion gene. No colonies displayed the <i>BCR-ABL1<\/i> transcript alone. These findings indicate that the <i>JAK2<\/i><sup>V617F<\/sup> mutation was the founding genetic alteration of the disease, followed by the acquisition of the <i>BCR-ABL1<\/i> chimeric oncogene. Our data support the hypothesis that a heterozygous <i>JAK2<\/i><sup>V617F<\/sup> clone may have favoured the bi-clonal nature of this myeloproliferative disorder, generating clones harbouring a second transforming genetic event.<\/jats:p>","DOI":"10.1159\/000496821","type":"journal-article","created":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T21:08:03Z","timestamp":1554844083000},"page":"261-267","source":"Crossref","is-referenced-by-count":7,"title":["Colony-Forming Cell Assay Detecting the Co-Expression of <b><i>JAK2<\/i><\/b><sup>V617F<\/sup> and <b><i>BCR-ABL1<\/i><\/b> in the Same Clone: A Case 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