{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T06:22:49Z","timestamp":1775542969366,"version":"3.50.1"},"reference-count":48,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Innate Immun"],"published-print":{"date-parts":[[2019]]},"abstract":"<jats:p>We characterized the dual oxidase (Duox) gene in the major Indian malaria vector &lt;i&gt;Anopheles stephensi&lt;\/i&gt;, which regulates the generation of reactive oxygen species. The AsDuox gene encodes for a 1,475-amino-acid transmembrane protein that contains an N-terminal noncytoplasmic heme peroxidase domain, a calcium-binding domain, seven transmembrane domains, and a C-terminal cytoplasmic NADPH domain. Phylogenetic analyses revealed that &lt;i&gt;A. stephensi&lt;\/i&gt; Duox protein is highly conserved and shares 97\u2013100% amino acid identity with other anopheline Duoxes. AsDuox is expressed in all the developmental stages of &lt;i&gt;A. stephensi&lt;\/i&gt; and the pupal stages revealed relatively higher expressions. The Duox gene is induced in &lt;i&gt;Plasmodium&lt;\/i&gt;-infected mosquito midguts, and RNA interference-mediated silencing of this gene suppressed parasite development through activation of the thioester-containing protein 1 pathway. We propose that this highly conserved anopheline Duox, being a &lt;i&gt;Plasmodium&lt;\/i&gt; agonist, is an excellent target to control malaria parasite development inside the insect host.<\/jats:p>","DOI":"10.1159\/000497417","type":"journal-article","created":{"date-parts":[[2019,3,29]],"date-time":"2019-03-29T22:00:32Z","timestamp":1553896832000},"page":"496-505","source":"Crossref","is-referenced-by-count":13,"title":["&lt;b&gt;&lt;i&gt;Anopheles stephensi&lt;\/i&gt;&lt;\/b&gt; Dual Oxidase Silencing Activates the Thioester-Containing Protein 1 Pathway to Suppress &lt;b&gt;&lt;i&gt;Plasmodium&lt;\/i&gt;&lt;\/b&gt; 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