{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T08:19:24Z","timestamp":1772612364815,"version":"3.50.1"},"reference-count":26,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Kidney Blood Press Res"],"published-print":{"date-parts":[[2019]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background\/Aims:&lt;\/i&gt;&lt;\/b&gt; Peritoneal fibrosis (PF) is a common complication in patients receiving long-term peritoneal dialysis, which results in damage to peritoneal functions. Epithelial-mesenchymal transition (EMT) is a key step in the early pathogenesis of PF. Increasing evidence has shown that signal transducer and activator of transcription 3 (STAT3) signaling pathway is involved in EMT and tissue fibrosis by interacting with distinct EMT-inducing molecules, including transforming growth factor (TGF)-\u03b2 and advanced glycation end products (AGEs). This study investigated the involvement of STAT3 in the PF process. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; We used high glucose-treated human peritoneal mesothelial cell line HMrSV5 as an in vitro model to expose the peritoneal mesothelial cells to high-glucose dialysate. Expression of EMT markers was detected by qRT-PCR. Accumulation of methylglyoxal (MGO) and AGEs in the culture supernatant were measured by enzyme-linked immunosorbent assay. Phosphorylation of STAT3 was assessed by Western blot. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Results showed that high glucose upregulated TGF-\u03b2, increased the productions of MGO and AGEs, and induced EMT in HMrSV5 cells. High glucose also activated the STAT3 pathway. STAT3 inhibitor reduced the high glucose-induced EMT, via reducing TGF-\u03b2 expression and repressing the accumulation of MGO and AGEs. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; Our results revealed a critical role for STAT3 signaling in high glucose-induced EMT in HMrSV5 cells, and suggested that inhibition of STAT3 might be a treatment for high glucose-induced fibrogenesis in PF.<\/jats:p>","DOI":"10.1159\/000498965","type":"journal-article","created":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T21:55:32Z","timestamp":1554328532000},"page":"179-187","source":"Crossref","is-referenced-by-count":17,"title":["Involvement of STAT3 Signaling in High Glucose-Induced Epithelial Mesenchymal Transition in Human Peritoneal Mesothelial Cell Line HMrSV5"],"prefix":"10.1159","volume":"44","author":[{"given":"Pei","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2019,4,3]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2013.02.027"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2016.03.040"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.2337\/db13-1606"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1042\/CS20140683"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-015-3597-5"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/14.6.1541"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3758"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.2337\/db17-0537"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-11-0271"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S81390"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2017.3098"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.200311"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006111292"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1172\/JCI39104"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/0272-6386(95)90610-X"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1172\/JCI36183"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.4149\/neo_2015_002"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00191.2002"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2840-13-89"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000077413.41276.17"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2016.01.009"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11951"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209281"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1797.2007.00881.x"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.TARG-11-A201"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25058"}],"container-title":["Kidney and Blood Pressure Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/498965","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/498965","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:23:34Z","timestamp":1745425414000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000498965"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":26,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,5,13]]}},"URL":"https:\/\/doi.org\/10.1159\/000498965","archive":["Portico"],"relation":{},"ISSN":["1420-4096","1423-0143"],"issn-type":[{"value":"1420-4096","type":"print"},{"value":"1423-0143","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]}}}