{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T19:02:10Z","timestamp":1789758130390,"version":"4.0.1"},"reference-count":32,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cardiology"],"published-print":{"date-parts":[[2019]]},"abstract":"<b><i>Objectives:<\/i><\/b> To explore the association between single-nucleotide polymorphisms (SNPs) in <i>MTHFR<\/i> and <i>APOE<\/i> and the risk of CAD and, more importantly, the severity of CAD and the profile of serum lipids, we performed a case-control study in a Chinese Han population. <b><i>Methods:<\/i><\/b> A total of 1,207 cases of consecutive CAD-suspected inpatients were recruited, and 406 CAD cases and 231 non-CAD controls were enrolled for the final analysis after screening for exclusion criteria. All subjects had undergone coronary angiography, and the severity of CAD was evaluated by 2 cardiologists according to the Gensini scores. The genotypes of <i>MTHFR<\/i> and <i>APOE<\/i>were detected using real-time PCR, and then verified by Sanger sequencing. Environmental risk factors, such as age, sex, smoking, alcohol consumption, hypertension, diabetes, dyslipidemia, and BMI were collected. Statistical analyses (the \u03c7<sup>2<\/sup> test, binary logistic regression analysis, and ordinal polytomous logistic regression analysis) were performed with SPSS v16.0. <b><i>Results:<\/i><\/b> The genotypes of<b><i><\/i><\/b>all the subjects included in the CAD and non-CAD groups in this study were successfully detected, with an agreement of 100% with Sanger sequencing. The distributions of genotypes<i> CT<\/i> and <i>TT<\/i> at <i>MTHFR C667T<\/i> were higher in CAD cases than in non-CAD controls (OR 1.99, 95% CI 1.34\u20132.95; OR 1.77, 95% CI 1.18\u20132.67; <i>p<\/i> &#x3c; 0.05), whereas genotype <i>AC<\/i> at <i>MTHFR A1298C<\/i>was lower in CAD cases (OR 0.71, 95% CI 0.50\u20131.02; <i>p<\/i> &#x3c; 0.05). A significant association was observed in genotypes <i>CT<\/i> and <i>TT<\/i> at <i>MTHFR C667T<\/i> and the risk of CAD (OR 1.44, 95% CI 1.27\u20133.67; OR 1.56, 95% CI 0.88\u20132.78;<i> p<\/i> &#x3c; 0.05). Both genotypes and alleles of <i>APOE<\/i> were comparable in the CAD cases and non-CAD controls (<i>p<\/i> &#x3e; 0.05). The genotype <i>TT<\/i> at <i>MTHFR C667T<\/i> and \u03b54<i>+<\/i> at <i>APOE<\/i> were more likely to be found in the CAD subgroup with a Gensini score \u226572 (<i>p<\/i> = 0.040 and <i>p<\/i> = 0.028, respectively). Meanwhile, in the patients with genotype <i>TT<\/i>,<i><\/i>a higher level of serum Hcy was detected, while genotype \u03b54+ patients possessed higher levels of serum apolipoprotein E (ApoE) and low-density lipoprotein cholesterol (LDL-C) than other genotypes. <b><i>Conclusion:<\/i><\/b> This study revealed that the SNP site of <i>MTHFR C667T<\/i>is associated<i><\/i>with the risk of CAD in this Chinese Han population. In addition, the genotypes of <i>TT<\/i> in <i>MTHFR C667T<\/i> and \u03b54<i>+<\/i>in <i>APOE<\/i> may increase the severity of CAD, and higher Hcy, LDL-C, and ApoE levels may be involved in this pathogenic process.<\/jats:p>","DOI":"10.1159\/000499866","type":"journal-article","created":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T21:00:34Z","timestamp":1559682034000},"page":"149-157","source":"Crossref","is-referenced-by-count":17,"title":["A Case-Control Study of the Association of the Polymorphisms of <b><i>MTHFR<\/i><\/b> and <b><i>APOE<\/i><\/b> with Risk Factors and the Severity of Coronary Artery Disease"],"prefix":"10.1159","volume":"142","author":[{"given":"Yan","family":"Long","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Tao","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan-Yuan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin-Ting","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Yu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Xuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2019,6,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.783"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-111212-133258"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.306566"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038\/jhg.2015.70"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5273(97)00211-8"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1517\/14622416.1.1.73"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.4330\/wjc.v8.i1.1"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(93)90139-4"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genom.1.1.507"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.114.013311"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17101733"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(80)90175-9"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199104253241701"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13988"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-015-0389-7"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.4103\/0366-6999.156803"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0181620"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2016.01.009"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2016.10.027"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2003.08.021"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000009044"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1159\/000343356"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000485"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jstrokecerebrovasdis.2015.11.041"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1177\/1076029618780344"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.14715\/cmb\/2017.63.6.13"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.14712\/18059694.2015.42"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1590\/S1415-47572010005000026"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3019"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.jstrokecerebrovasdis.2017.05.048"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2018.08.004"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2362.2002.00983.x"}],"container-title":["Cardiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/499866","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/499866","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:31:59Z","timestamp":1745425919000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000499866"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":32,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2019,7,10]]},"published-print":{"date-parts":[[2019,7,16]]}},"URL":"https:\/\/doi.org\/10.1159\/000499866","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.8224130","asserted-by":"subject"}]},"ISSN":["0008-6312","1421-9751"],"issn-type":[{"value":"0008-6312","type":"print"},{"value":"1421-9751","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]}}}