{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:28:27Z","timestamp":1792114107366,"version":"4.4.0"},"reference-count":26,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neonatology"],"published-print":{"date-parts":[[2019]]},"abstract":"<b><i>Background:<\/i><\/b> Bronchopulmonary dysplasia (BPD) is a common cause of abrupted lung development after preterm birth. BPD may lead to increased rehospitalization, more severe and frequent respiratory infections, and life-long reduced lung function. The gene regulation in lungs with BPD is complex, with various genetic and epigenetic factors involved. <b><i>Objectives:<\/i><\/b> The aim of this study was to examine the regulatory relation between gene expression and the epigenome (DNA methylation) relevant for the immune system after hyperoxia followed by a recovery period in air using a mouse model of BPD. <b><i>Methods:<\/i><\/b> Newborn mice pups were subjected to an immediate hyperoxic condition from birth and kept at 85% O<sub>2<\/sub> levels for 14 days followed by a 14-day period in room air. Next, mice lung tissue was used for RNA and DNA extraction with subsequent microarray-based assessment of lung transcriptome and supplementary methylome analysis. <b><i>Results:<\/i><\/b><i><\/i>The immune system-related transcriptome<b><i><\/i><\/b>regulation was affected in mouse lungs after hyperoxia. A high proportion of genes relevant in the immune system exhibited significant expression alterations, e.g., B cell-specific genes central to the cytokine-cytokine receptor interaction, the PI3K-AKT, and the B cell receptor signaling pathways. The findings were accompanied by significant DNA hypermethylation observed in the PI3K-AKT pathway and immune system-relevant genes. <b><i>Conclusions:<\/i><\/b> Oxygen damage could be partly responsible for the increased susceptibility and abnormal response to respiratory viruses and infections seen in premature babies with BPD through dysregulated genes.<\/jats:p>","DOI":"10.1159\/000501461","type":"journal-article","created":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T21:00:34Z","timestamp":1566939634000},"page":"269-277","source":"Crossref","is-referenced-by-count":23,"title":["Immune System Regulation Affected by a Murine Experimental Model of Bronchopulmonary Dysplasia: Genomic and Epigenetic Findings"],"prefix":"10.1159","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2453-4528","authenticated-orcid":false,"given":"Cecilie","family":"Revhaug","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslaw","family":"Bik-Multanowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Zasada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u00a0Gro\u00a0W.","family":"Rognlien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara-Cecilie","family":"G\u00fcnther","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teofila","family":"Ksi\u0105zek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Madetko-Talowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Szewczyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Grabowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3017-0348","authenticated-orcid":false,"given":"Przemko","family":"Kwinta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacek\u00a0J.","family":"Pietrzyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars\u00a0O.","family":"Baumbusch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3166-5254","authenticated-orcid":false,"given":"Ola\u00a0D.","family":"Saugstad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2019,8,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1700827"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000108191.13799.09"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.163.7.2011060"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2011-0583"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00039513"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1097\/MOP.0b013e328346577f"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/pr.2016.201"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e318184edeb"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.04.566"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.07.037"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1053\/j.semperi.2018.09.009"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01392.2009"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2014.12.006"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.8.1123"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000202"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.07.029"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1038\/nri2901"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.56.4.317"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106392"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00233.2014"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1186\/s40348-016-0051-9"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.3390\/v10080392"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1038\/pr.2015.155"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-016-2534-3"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.5.2731"}],"container-title":["Neonatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/501461","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/501461","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:42:05Z","timestamp":1745422925000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000501461"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":26,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2019,10,8]]},"published-pri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