{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:50:30Z","timestamp":1772585430743,"version":"3.50.1"},"reference-count":24,"publisher":"S. Karger AG","issue":"4","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dig Surg"],"published-print":{"date-parts":[[2020]]},"abstract":"<b><i>Background:<\/i><\/b> Oral antibiotics, such as ciprofloxacin (CFX), are widely used for the treatment of acute and chronic pouchitis. Most bacterial mutations that confer quinolone resistance are at Ser-83 and Asp-87 in the <i>gyrA<\/i> gene and Ser-80 and Glu-84 in the <i>parC<\/i> gene. <b><i>Methods:<\/i><\/b> We obtained 51 stool samples from 43 patients who were diagnosed with ulcerative colitis and underwent ileal pouch-anal anastomosis. Patients were divided into 2 groups: 13 patients with CFX treatment of pouchitis and 30 patients without pouchitis. After extraction of fecal DNA, the amount of <i>Escherichia coli 16S rRNA<\/i>, <i>gyrA<\/i>, and <i>parC<\/i> gene DNA were measured using real-time polymerase chain reaction (PCR). Possible mutations at <i>gyrA<\/i> 83 and 87 and at <i>parC<\/i> 80 and 84 were investigated by PCR cloning and sequencing, and mutation rates were quantified by rapid PCR-restriction fragment length polymorphism. <b><i>Results:<\/i><\/b> Samples from both CFX-treated and -untreated patients had comparable levels of <i>gyrA<\/i> and <i>parC<\/i> gene DNA. Nucleic acid and amino acid mutations were identified at <i>gyrA<\/i> 83 and 87, and at <i>parC<\/i> 80 and 84. We successfully quantified mutation rates at <i>gyrA<\/i> 83 and 87, and at <i>parC<\/i> 84, all of which were significantly higher in samples from CFX-treated patients (70, 84, and 38%) than from CFX-untreated patients (13, 11, and 5%). <b><i>Conclusion:<\/i><\/b> <i>E. coli<\/i> in patient pouches may have mutations in their <i>gyrA<\/i> and <i>parC<\/i> genes that produce CFX resistance. Mutation rates of these genes were significantly higher in samples from CFX-treated patients. This study contributes to understanding the decrease and loss of CFX effectiveness against pouchitis.<\/jats:p>","DOI":"10.1159\/000504750","type":"journal-article","created":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T22:00:31Z","timestamp":1584655231000},"page":"321-330","source":"Crossref","is-referenced-by-count":7,"title":["Increased Quinolone-Resistant Mutations of <b><i>gyrA<\/i><\/b> and <b><i>parC<\/i><\/b> Genes after Pouchitis Treatment with Ciprofloxacin"],"prefix":"10.1159","volume":"37","author":[{"given":"Kouhei","family":"Fukushima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Kohyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2020,3,17]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.13905"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/s0167-4781(98)00127-4"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1179\/joc.2011.23.3.145"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.11.4772"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.11.4767"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.25.11801"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1177\/0300060513475965"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/18.supplement_d.21"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1128\/aac.40.2.491"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1128\/aac.42.10.2661"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2007.3"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000087107"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2008.12.003"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-012-2165-9"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-007-2083-3"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1007\/s10350-004-6652-8"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2013.06.019"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.03049-12"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00016-09"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00147-08"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2012.58"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/s0732-8893(02)00382-6"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01355"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-1-257"}],"container-title":["Digestive Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/504750","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/504750","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:14:19Z","timestamp":1745424859000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000504750"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":24,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,6,30]]},"published-print":{"date-parts":[[2020,7,3]]}},"URL":"https:\/\/doi.org\/10.1159\/000504750","archive":["Portico"],"relation":{"is-supplemented-by":[{"id-type":"doi","id":"10.6084\/m9.figshare.11993709","asserted-by":"subject"}]},"ISSN":["0253-4886","1421-9883"],"issn-type":[{"value":"0253-4886","type":"print"},{"value":"1421-9883","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020]]}}}