{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T04:55:36Z","timestamp":1789102536045,"version":"build-2803163510"},"reference-count":22,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T00:00:00Z","timestamp":1576713600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T00:00:00Z","timestamp":1576713600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2020]]},"abstract":"<b><i>Introduction:<\/i><\/b> The relationship of parasite infections and promotion or protection from allergy and asthma is controversial. Currently, over 1.5 billion people are infected with parasites worldwide, and <i>Ascaris lumbricoides<\/i> is the most frequent soil-transmitted helminth. <b><i>Objectives:<\/i><\/b> To evaluate the biological activity of recombinant <i>A. lumbricoides<\/i> tropomyosin and investigate IgE cross-reactive responses to tropomyosins by means of microarray methodology for the detection of sensitization to allergen components. <b><i>Methods:<\/i><\/b> Forty patients 12\u201375 years of age (25 males) with asthma and\/or rhinitis and 10 nonallergic control subjects participated in this study. All patients presented positive skin tests to cockroach extracts and underwent skin prick testing (SPT) with recombinant (r) tropomyosins rPer a 7 from <i>Periplaneta americana<\/i> and rAsc l 3 from <i>A. lumbricoides<\/i>, at 10 \u03bcg\/mL. IgE to cockroach and parasite tropomyosins were measured by chimeric ELISA and ImmunoCAP-ISAC, and total IgE was quantitated by ImmunoCAP. Agreement of results was assessed by \u03ba statistics. <b><i>Results:<\/i><\/b> Recombinant <i>A. lumbricoides<\/i> showed biological activity, inducing positive skin tests in 50% patients with asthma and\/or rhinitis. IgE to cockroach and parasite tropomyosins were detected in 55\u201362% of patients. There was good-to-excellent agreement of results of SPT and IgE measurements by ELISA and ImmunoCAP-ISAC, with \u03ba indices of 0.66\u20130.95. No skin test reactivity or IgE antibodies to tropomyosins were found in nonallergic individuals. <b><i>Conclusions:<\/i><\/b> Our results suggest that IgE responses to tropomyosin from <i>A. lumbricoides<\/i> may enhance reactivity to homologous allergens upon exposure by inhalation or ingestion, promoting allergic reactions and asthma, or increasing the severity of these clinical conditions.<\/jats:p>","DOI":"10.1159\/000504982","type":"journal-article","created":{"date-parts":[[2019,12,19]],"date-time":"2019-12-19T22:00:33Z","timestamp":1576792833000},"page":"221-227","source":"Crossref","is-referenced-by-count":13,"title":["Parasite Infections, Allergy and Asthma: A Role for Tropomyosin in Promoting Type 2 Immune Responses"],"prefix":"10.1159","volume":"181","author":[{"given":"Ana\u00a0Carolina\u00a0A.F.","family":"Sousa-Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3478-7414","authenticated-orcid":false,"given":"Adriana\u00a0S.","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana\u00a0Beatriz\u00a0R.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle\u00a0C.R.","family":"Barbosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1019-3654","authenticated-orcid":false,"given":"Davi\u00a0C.","family":"Aragon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria\u00a0S.F.","family":"Sales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.\u00a0Karla","family":"Arruda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2019,12,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.09.005"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12589"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2019.03.009"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12588"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.16-0348"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2016.12.002"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12595"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1186\/s40413-015-0058-z"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12513"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.12.1147"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1159\/000321106"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1159\/000346318"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2003.00167.x"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-017-0694-1"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.18176\/jiaci.0378"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2018.04.035"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2018.08.008"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0000000000000529"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0000000000000568"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2009.03433.x"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2012.04063.x"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1159\/000431360"}],"container-title":["International Archives of Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/504982","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/504982","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:16:40Z","timestamp":1745425000000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000504982"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,19]]},"references-count":22,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,2,26]]},"published-print":{"date-parts":[[2020,2,26]]}},"URL":"https:\/\/doi.org\/10.1159\/000504982","archive":["Portico"],"relation":{},"ISSN":["1018-2438","1423-0097"],"issn-type":[{"value":"1018-2438","type":"print"},{"value":"1423-0097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,19]]}}}