{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T23:31:27Z","timestamp":1782603087678,"version":"3.54.5"},"reference-count":0,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am J Nephrol"],"published-print":{"date-parts":[[2020]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background and Aim:&lt;\/i&gt;&lt;\/b&gt; Primary membranous nephropathy (pMN) is the most common cause of nephrotic syndrome in adults. Recent studies suggested that immunization of DBA\/1 mice with the main antigen of antiglomerular basement membrane disease (GBM) disease, \u03b13(IV)NC1, could lead to MN lesions. This study aimed to explore the pathogenic epitopes for mouse MN. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Twenty-four linear peptides were synthesized spanning human \u03b13(IV)NC1. Male DBA\/1 mice aged 6\u20138 weeks were immunized with the peptides 200 \u03bcg\/mouse in Freund\u2019s complete adjuvant subcutaneously and boosted 3 times with the peptides in Freund\u2019s incomplete adjuvant in weeks 3, 5, and 7. The blood and 24-h urine samples were assessed every 2 weeks. The kidneys were examined when the mice were sacrificed at 18 weeks. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; All the mice immunized with human \u03b13(IV)NC1 and the 24 peptides produced circulating antibodies against the immunogens at 2 weeks and achieved the maximum titers at 8 weeks. About 5\/6 (83%) mice immunized with \u03b13(IV)NC1 and (3\/6) 50% of the mice immunized with peptide 23 (\u03b13&lt;sub&gt;141\u2013154&lt;\/sub&gt;) showed proteinuria at 8\u201310 weeks and increased continuously. The kidneys showed granular depositions of IgG, C3, and C5b-9 along the glomerular capillary loops. The major IgG subclass was IgG1 (equivalent to human IgG4). GBM thickening with the formation of spikes and subepithelial electron-dense deposits were observed under electron microscope. &lt;b&gt;&lt;i&gt;Conclusion:&lt;\/i&gt;&lt;\/b&gt; The linear peptide of \u03b13&lt;sub&gt;141\u2013154&lt;\/sub&gt; could induce clinical and histopathological features of MN in DBA\/1 mice, which might give clues to the mechanism of MN in combination with anti-GBM disease.<\/jats:p>","DOI":"10.1159\/000505443","type":"journal-article","created":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T22:00:31Z","timestamp":1578693631000},"page":"99-107","source":"Crossref","is-referenced-by-count":8,"title":["Epitope Mapping of Human \u03b13(IV)NC1-Induced Membranous Nephropathy in Mice"],"prefix":"10.1159","volume":"51","author":[{"given":"Jia","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-hui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2020,1,10]]},"container-title":["American Journal of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/505443","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/505443","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:39:05Z","timestamp":1745422745000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000505443"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,2,12]]},"published-print":{"date-parts":[[2020,2,12]]}},"URL":"https:\/\/doi.org\/10.1159\/000505443","archive":["Portico"],"relation":{},"ISSN":["0250-8095","1421-9670"],"issn-type":[{"value":"0250-8095","type":"print"},{"value":"1421-9670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020]]}}}