{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T04:25:25Z","timestamp":1745468725345,"version":"3.40.4"},"reference-count":38,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytogenet Genome Res"],"published-print":{"date-parts":[[2020]]},"abstract":"<jats:p>DCC netrin 1 receptor (DCC) affects the structure and function of the dopamine circuitry, which in turn affects the susceptibility to developing addiction. In a previous study, we found that single nucleotide polymorphism (SNP) rs12607853 in the 3\u2032 untranslated region (3\u2032-UTR) of &lt;i&gt;DCC&lt;\/i&gt; was significantly associated with heroin addiction. In the current study, we first used bioinformatics prediction to identify the &lt;i&gt;DCC&lt;\/i&gt; rs12607853 C allele as a potential hsa-miR-422a and hsa-miR-378c target site. We then used vector construction and dual-luciferase reporter assays to investigate the targeting relationship of &lt;i&gt;DCC&lt;\/i&gt; rs12607853 with hsa-miR-422a and hsa-miR-378c. The dual-luciferase reporter gene assay confirmed that the C allele of rs12607853 in combination with hsa-miR-422a led to repressed dual-luciferase gene expression. Moreover, gene expression assays disclosed that hsa-miR-422a inhibited DCC expression at both the mRNA and protein levels. We also found that morphine inhibited the expression of hsa-miR-422a but increased the expression of &lt;i&gt;DCC&lt;\/i&gt; mRNA, and this change in the expression of hsa-miR-422a could not be reversed by naloxone, which suggested that the role of DCC in opioid addiction might be regulated by hsa-miR-422a. In summary, this study improves our understanding of the role of hsa-miR-422a and identifies the genetic basis of rs12607853, which might contribute to the discovery of new biomarkers or therapeutic targets for opioid addiction.<\/jats:p>","DOI":"10.1159\/000506031","type":"journal-article","created":{"date-parts":[[2020,2,24]],"date-time":"2020-02-24T22:00:34Z","timestamp":1582581634000},"page":"63-71","source":"Crossref","is-referenced-by-count":2,"title":["microRNA-422a Inhibits &lt;b&gt;&lt;i&gt;DCC&lt;\/i&gt;&lt;\/b&gt; Expression in a Manner Dependent on SNP 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