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Children under the age of 2 years, hospitalized for bronchiolitis in the pediatric clinic of a tertiary hospital in northern Greece, were tested for RSV infection during two RSV seasons (2016\u20132017 and 2017\u20132018). RSV was detected in 37 of 71 (52.1%) patients, most of them younger than 6 months. Both RSV subtypes were detected \u2013 RSV-A (54.1%) and RSV-B (45.9%) \u2013 with predominance of RSV-A during the 2016\u20132017 and RSV-B during the 2017\u20132018 season. RSV-A and RSV-B sequences clustered within the ON1 and BA genotypes, respectively. Compared to the prototype strains, several amino acid substitutions were observed in the duplication region of the G gene. 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