{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T15:43:12Z","timestamp":1772638992157,"version":"3.50.1"},"reference-count":24,"publisher":"S. Karger AG","issue":"1-2","license":[{"start":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T00:00:00Z","timestamp":1595289600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T00:00:00Z","timestamp":1595289600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Introduction:&lt;\/i&gt;&lt;\/b&gt; Homeostasis of cholesterol is crucial for cellular function, and dysregulated cholesterol biosynthesis is a metabolic event that can lead to hepatic and cardiovascular abnormalities. &lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; The aim of this study was to investigate the effects and mechanisms of domain-associated protein (Daxx) and androgen receptor (AR) on intracellular cholesterol synthesis. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; HepG2 cells were transfected with pCDNA3.1(+)\/Daxx plasmid or treated with testosterone propionate to observe the effects of Daxx and AR on intracellular cholesterol levels. Co-immunoprecipitation experiments were performed to identify the interaction between Daxx and AR and to explore the regulatory effects of this interaction on cholesterol synthesis. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Our experiments showed that AR promoted cholesterol synthesis and accumulation by activating sterol-regulatory element-binding protein isoform 2. AR-induced cholesterol synthesis was inhibited by Daxx; however, the expression of AR was not affected. Further studies demonstrated the existence of direct binding between Daxx and AR and this interaction was required to suppress AR activity. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; The Daxx-mediated antagonism of AR depicts a more complete picture as to how Daxx regulates intracellular cholesterol level and provides a new target for treatment of atherosclerosis. <\/jats:p>","DOI":"10.1159\/000506488","type":"journal-article","created":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T21:01:05Z","timestamp":1595365265000},"page":"29-36","source":"Crossref","is-referenced-by-count":6,"title":["Direct Interaction of Daxx and Androgen Receptor Is Required for Their Regulatory Activity in Cholesterol Biosynthesis"],"prefix":"10.1159","volume":"106","author":[{"given":"Tian-Ping","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shao-Wei","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Zuo","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong-Mei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si-Yu","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue-Jiao","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duan-Fang","family":"Liao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian-Xiong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin-Hui","family":"Tuo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2020,7,21]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1093\/milmed\/usx005"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.118.008819"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.31149"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1192\/bjp.169.6.682"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jad.2010.02.111"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1186\/s12991-017-0144-4"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.6b00342"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M039339"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.2131\/jts.44.481"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2017.11.007"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-017-5961-9"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/s0303-7207(03)00205-3"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1210\/me.2005-0479"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1808834116"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.4161\/cc.8.10.8379"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1515\/cmble-2015-0048"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.14.435"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2010.166"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.14027"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1186\/s12944-018-0905-3"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v4.i6.184"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.C066712"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.227082"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2005.12.002"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/506488","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/506488","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:51:05Z","timestamp":1745423465000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000506488"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,21]]},"references-count":24,"journal-issue":{"issue":"1-2","published-online":{"date-parts":[[2021,2,4]]},"published-print":{"date-parts":[[2021,2,9]]}},"URL":"https:\/\/doi.org\/10.1159\/000506488","archive":["Portico"],"relation":{},"ISSN":["0031-7012","1423-0313"],"issn-type":[{"value":"0031-7012","type":"print"},{"value":"1423-0313","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,21]]}}}