{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T03:51:01Z","timestamp":1792900261234,"version":"4.5.0"},"reference-count":24,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Pharmacol Physiol"],"published-print":{"date-parts":[[2020]]},"abstract":"<b><i>Background\/Aim:<\/i><\/b> Hydrogen sulfide (H<sub>2<\/sub>S) has been found to act as a physiological intercellular messenger to regulate cell survival. In this study, we evaluated whether H<sub>2<\/sub>S could promote cell proliferation and melanin synthesis in human epidermal melanocytes (HEMs). <b><i>Methods:<\/i><\/b> Primary HEMs were cocultured with sodium hydrosulfide (NaHS, the most widely used H<sub>2<\/sub>S donor) or endogenously overexpressed with cystathionine-\u03b3-lyase (CSE) gene, which is the most predominant H<sub>2<\/sub>S-producing enzyme. Then, cell viability, intracellular melanin content, tyrosinase (TYR) activity, and expression of microphthalmia-associated transcription factor (MITF), TYR, together with TYR-related protein 1 (TRP-1) in both transcript and protein levels, were detected. <b><i>Results:<\/i><\/b> We first confirmed that NaHS (10\u2013100 \u03bcm) increased cell viability, intracellular melanin content, and TYR activity in a dose-dependent manner. Then, we found that endogenous H<sub>2<\/sub>S production also promoted cell proliferation, intracellular melanin content, and TYR activity. In addition, we observed the mRNA and protein expression of MITF, TYR, and TRP-1 was significantly up-regulated after NaHS treatment and CSE gene transfection. <b><i>Conclusions:<\/i><\/b> This study demonstrates that H<sub>2<\/sub>S promotes cell proliferation and melanin synthesis in HEMs, which indicates pharmacologic regulation of H<sub>2<\/sub>S may be potential treatment for skin disorders caused by loss of melanocytes or dysfunction of melanogenesis. <\/jats:p>","DOI":"10.1159\/000506818","type":"journal-article","created":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T21:04:14Z","timestamp":1591131854000},"page":"61-68","source":"Crossref","is-referenced-by-count":12,"title":["Hydrogen Sulfide Promotes Cell Proliferation and Melanin Synthesis in Primary Human Epidermal Melanocytes"],"prefix":"10.1159","volume":"33","author":[{"given":"Jiayi","family":"Ying","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuxiu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengfeng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leihong","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2020,6,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.08.005"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R700026200"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00044.2003"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099593"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1523\/jneurosci.16-03-01066.1996"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.4349"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1134\/s1990519x11060046"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2008.2253"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20040243"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000013065"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.01.047"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12312"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2009.08.003"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1586\/ecp.10.131"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2019.07.092"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcha.2015.01.010"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1242\/dev.106567"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5054-y"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-202x.2001.00010.x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2016.08.004"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-14-166"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmx072"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0705891104"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-05457-z"}],"container-title":["Skin Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/506818","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/506818","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T15:54:26Z","timestamp":1745423666000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000506818"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":24,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,6,12]]},"published-print":{"date-parts":[[2020,6,16]]}},"URL":"https:\/\/doi.org\/10.1159\/000506818","archive":["Portico"],"relation":{},"ISSN":["1660-5527","1660-5535"],"issn-type":[{"value":"1660-5527","type":"print"},{"value":"1660-5535","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020]]}}}