{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T03:31:52Z","timestamp":1747193512214,"version":"3.40.5"},"reference-count":35,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Cytologica"],"published-print":{"date-parts":[[2020]]},"abstract":"<b><i>Introduction\/Objective:<\/i><\/b> Differently from other digestive malignancies, gastric cancer (GC) pathobiology is still little known and understood. Recently, cytopathology and molecular biology on gastric juice\/gastric lavage (GJ\/GL) of GC patients have provided novel and interesting results in terms of screening, diagnosis, prognosis, and therapy. However, entertaining cytologic examination and molecular test as a unified solo-run test is previously unreported. Our aim was to assess the new parameter \u201cGL Ca 72.4\u201d for GC patients. <b><i>Methods:<\/i><\/b> Between April 2012 and July 2013, GJ\/GL obtained from 37 surgical GC patients were tested for the presence\/absence (GL1\/GL0) of exfoliated malignant cells along with the intragastric concentration of Ca 72.4 (normal value &#x3c;6.49 ng\/mL: Ca 72.4n; elevated level \u22656.49 ng\/mL: Ca 72.4+). <b><i>Results:<\/i><\/b> At a median follow-up of 79.3 months, all the GC alive patients were \u201cGL0 Ca 72.4n.\u201d The \u201cGL1 Ca 72.4+\u201d parameter, in comparison with GL0 Ca 72.4n, strongly correlated with deeper tumor invasion (<i>p<\/i> = 0.027), severe nodal metastasis (<i>p<\/i> = 0.012), worst metastatic node ratio (<i>p<\/i> = 0.041), higher number of metastatic lymph nodes (30 vs. 20 nodes, <i>p<\/i> = 0.014), angiolymphatic invasion (<i>p<\/i> = 0.044), advanced stage (<i>p<\/i> = 0.034), and adjuvant therapy (<i>p<\/i> = 0.044). The Kaplan-Meier model showed that GL1 Ca 72.4+ subjects had shorter overall survival (OS) than GL0 Ca 72.4n cases (9.7 vs. 43.2 months, respectively, <i>p<\/i> = 0.042). At univariate analysis, the GL1 Ca 72.4+ parameter resulted a significant prognostic factor for OS (<i>p<\/i> = 0.023). <b><i>Conclusions:<\/i><\/b> The combined cyto-molecular parameter \u201cGL1 Ca 72.4+\u201d appears to be a strong indicator of aggressive tumor behavior and a significant prognostic factor of poor survival for GC patients. <\/jats:p>","DOI":"10.1159\/000508019","type":"journal-article","created":{"date-parts":[[2020,6,11]],"date-time":"2020-06-11T21:00:33Z","timestamp":1591909233000},"page":"563-571","source":"Crossref","is-referenced-by-count":1,"title":["Combined Analysis of Intragastric Malignant Exfoliation and Ca 72.4 Concentration in Stomach Adenocarcinoma: The \u201cGL1 Ca 72.4\u201d Parameter"],"prefix":"10.1159","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9324-3487","authenticated-orcid":false,"given":"Edoardo","family":"Virgilio","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrico","family":"Giarnieri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria 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