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Karger AG","issue":"1","license":[{"start":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T00:00:00Z","timestamp":1600041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T00:00:00Z","timestamp":1600041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int Arch Allergy Immunol"],"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background:&lt;\/i&gt;&lt;\/b&gt; The association among the IgE responses to prevailing groups of house dust mite (HDM) allergens in the concurrent asthma phenotypes has not been determined. &lt;b&gt;&lt;i&gt;Objective:&lt;\/i&gt;&lt;\/b&gt; The aim of the present study lays on a component-resolved diagnosis (CRD) model to investigate the mite molecular signature in subjects with type-2 inflammation asthma. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; We selected patients showing a clinically relevant sensitization to HDMs with moderate-to-severe persistent asthma. Skin prick test (SPT) with standardized mite extracts, a broad customized CRD serum sIgE panel including 9 &lt;i&gt;Dermatophagoides pteronyssinus&lt;\/i&gt; allergens and the related protein allergenic characterization, was investigated in all serum samples. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; Ninety out of 93 (96.77%) patients with a positive SPT to HDM showed a concordant sIgE (\u22650.35 kU&lt;sub&gt;A&lt;\/sub&gt;\/L) to the crude extract of &lt;i&gt;D. pteronyssinus&lt;\/i&gt;. Major allergens (Der p 2, Der p 23, and Der p 1) were present in &amp;#x3e;70% of all subjects, with mid-tier allergens (Der p 5, Der p 7, and Der p 21) reaching up to 51% in the present cohort. A complex pleomorphic repertoire of HDM molecules recognized by IgE was depicted, including 38 distinct profiles. &lt;b&gt;&lt;i&gt;Conclusions and Clinical Relevance:&lt;\/i&gt;&lt;\/b&gt; The proposed CRD panel approach, containing the most prevalent HDM allergens, appeared to be sufficient to obtain a precise &lt;i&gt;D. pteronyssinus&lt;\/i&gt; molecular diagnosis in asthmatics with a climate-dependent high-mite allergen exposure and coexisting sensitization. A dominant role of both major and mid-tier allergens has been confirmed in moderate and severe persistent asthmatics with the preponderant Th2-high endotype. <\/jats:p>","DOI":"10.1159\/000510118","type":"journal-article","created":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T21:00:33Z","timestamp":1600117233000},"page":"21-31","source":"Crossref","is-referenced-by-count":12,"title":["Mite Molecular Profile in the Th2-Polarized Moderate-to-Severe Persistent Asthma Endotype Subjected to High Allergen 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