{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:45:19Z","timestamp":1790829919615,"version":"4.1.0"},"reference-count":8,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dermatology"],"published-print":{"date-parts":[[2021]]},"abstract":"<b><i>Background:<\/i><\/b> Diagnosis of extrapulmonary histoplasmosis in HIV seronegative and immunocompetent patients is often challenging, so a high index of suspicion is required. Cutaneous manifestation of infection shows a wide spectrum of lesions including erythematous plaques; maculopapules; crusted, verrucous, or desquamative papules and nodules; abscesses; and mucocutaneous ulcers among others. Due to the variations in its clinical presentation, histopathology plays a very important role in the detection of spores and the confirmation of diagnosis. <b><i>Objectives:<\/i><\/b> The aim of our study was to analyze clinicopathological characteristics of cutaneous manifestations of biopsy-proven histoplasmosis in HIV seronegative individuals. We also examined the utility of Fite stain for the diagnosis of <i>Histoplasma capsulatum<\/i> on tissue biopsy sections. <b><i>Methods:<\/i><\/b> This was a retrospective, observational study on 7 patients who were HIV seronegative and clinically manifested with isolated cutaneous lesions or disseminated disease. Skin biopsy from the lesions was performed on all 7 patients. In addition to H&amp;E staining and special stains for detecting fungus, Fite staining was performed on all of the cases to study its utility in detecting <i>H. capsulatum<\/i> spores. <b><i>Results:<\/i><\/b> The skin lesions were widely disseminated in all patients and the most common cutaneous lesions were papules, present in all 7 patients. On review of the H&amp;E-stained slides, the most common pattern was histiocytic lobular panniculitis-like infiltrate observed in 4 cases. Fite stain highlighted the yeast as magenta-colored spores on a blue background in all cases, except for 1 with a granulomatous pattern. <b><i>Conclusion:<\/i><\/b> A primary cutaneous manifestation of <i>H. capsulatum<\/i> infection in non-HIV-infected individuals is extremely rare. Fite stain could aid in differentiating the spores of <i>H. capsulatum<\/i> from those of other fungi, <i>Cryptococcus<\/i> and <i>Candida<\/i> in particular.<\/jats:p>","DOI":"10.1159\/000513399","type":"journal-article","created":{"date-parts":[[2021,3,29]],"date-time":"2021-03-29T17:06:12Z","timestamp":1617037572000},"page":"934-939","source":"Crossref","is-referenced-by-count":12,"title":["Cutaneous Histoplasmosis in HIV Seronegative Patients: A Clinicopathological Analysis"],"prefix":"10.1159","volume":"237","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7003-5952","authenticated-orcid":false,"given":"Arvind","family":"Ahuja","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minakshi","family":"Bhardwaj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poojan","family":"Agarwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2021,2,24]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S1130-1406(05)70027-9"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0b013e31822fd00a"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.3109\/13693786.2013.777166"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2005.73.576"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00027-06"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00053-10"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.4132\/jptm.2017.07.11"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s40475-014-0017-8"}],"container-title":["Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/513399","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/513399","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T12:14:09Z","timestamp":1745410449000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000513399"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":8,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,11,2]]},"published-print":{"date-parts":[[2021,11,2]]}},"URL":"https:\/\/doi.org\/10.1159\/000513399","archive":["Portico"],"relation":{},"ISSN":["1018-8665","1421-9832"],"issn-type":[{"value":"1018-8665","type":"print"},{"value":"1421-9832","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021]]}}}