{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:52:15Z","timestamp":1787017935163,"version":"build-2736575974"},"reference-count":41,"publisher":"S. Karger AG","issue":"7-8","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pharmacology"],"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Background\/Aims:&lt;\/i&gt;&lt;\/b&gt; Daphnetin (7,8-dihydroxycoumarin, DAP) exhibits various bioactivities, such as anti-inflammatory and antioxidant activities. However, the role of DAP in myocardial ischaemia\/reperfusion (I\/R) injury and I\/R-related arrhythmia is still uncertain. This study aimed to investigate the mechanisms underlying the effects of DAP on myocardial I\/R injury and electrophysiological properties in vivo and in vitro. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; Myocardial infarct size was measured by triphenyltetrazolium chloride staining. Cardiac function was assessed by echocardiographic and haemodynamic analyses. The levels of creatine kinase-MB, lactate dehydrogenase, malondialdehyde, superoxide dismutase, interleukin-6 (IL-6), and tumour necrosis factor-alpha (TNF-\u03b1) were detected using commercial kits. Apoptosis was measured by terminal deoxynucleotidyl-transferase-mediated dUTP nick-end labelling staining and flow cytometry. The viability of H9c2 cells was determined by the Cell Counting Kit-8 assay. In vitro, the levels of IL-6 and TNF-\u03b1 were measured by quantitative PCR. The expression levels of proteins associated with apoptosis, inflammation, and the Toll-like receptor 4\/myeloid differentiation factor 88\/nuclear factor kappa B (TLR4\/MyD88\/NF-\u03baB) signalling pathway were detected by Western blot analysis. The RR, PR, QRS, and QTc intervals were assessed by surface ECG. The 90% action potential duration (APD&lt;sub&gt;90&lt;\/sub&gt;), threshold of APD alternans, and ventricular tachycardia inducibility were measured by the Langendorff perfusion technique. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; DAP preconditioning decreased myocardial I\/R injury and hypoxia\/reoxygenation (H\/R) injury in cells. DAP preconditioning improved cardiac function after myocardial I\/R injury. DAP preconditioning also suppressed apoptosis, attenuated oxidative stress, and inhibited inflammatory responses in vivo and in vitro. Furthermore, DAP preconditioning decreased the susceptibility to ventricular arrhythmia after myocardial I\/R. Finally, DAP preconditioning inhibited the expression of TLR4, MyD88, and phosphorylated NF-\u03baB (p-NF-\u03baB)\/P65 in mice subjected to I\/R and cells subjected to H\/R. &lt;b&gt;&lt;i&gt;Conclusions:&lt;\/i&gt;&lt;\/b&gt; DAP preconditioning protected against myocardial I\/R injury and decreased susceptibility to ventricular arrhythmia by inhibiting the TLR4\/MyD88\/NF-\u03baB signalling pathway.<\/jats:p>","DOI":"10.1159\/000513631","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T17:03:36Z","timestamp":1619456616000},"page":"369-383","source":"Crossref","is-referenced-by-count":13,"title":["Daphnetin Preconditioning Decreases Cardiac Injury and Susceptibility to Ventricular Arrhythmia following Ischaemia-Reperfusion through the TLR4\/MyD88\/NF-\u039ab Signalling Pathway"],"prefix":"10.1159","volume":"106","author":[{"given":"Fan","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobo","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyi","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Shuai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangji","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dajun","family":"Quan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7117-8939","authenticated-orcid":false,"given":"Xuejun","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2021,4,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8191.1993.tb01332.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8191.1993.tb01316.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071667"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2507"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.4330\/wjc.v10.i9.74"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101292"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116560"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.2174\/1381612043382710"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2014.04.005"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2816056"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2018.11.025"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11267"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.04.195"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.8208"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-013-0392-7"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.305348"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1080\/07853890701374677"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00024.2007"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvs018"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.02.029"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/s0008-6363(01)00434-5"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcm.2005.06.005"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2019.05.003"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2003.10.011"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/s0735-1097(02)01738-2"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-016-0931-4"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42465-7"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb09255.x"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2003.tb00225.x"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2308"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28854"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.280.5.H2313"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1016\/j.jelectrocard.2007.05.020"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00699.2005"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000187456.06162.cb"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.hrthm.2008.06.017"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.hjc.2017.12.009"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-05379-w"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2014.08.012"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2003.11.036"},{"key":"ref41","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000112575.66565.84"}],"container-title":["Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/513631","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/513631","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[202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