{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:20:48Z","timestamp":1790036448290,"version":"4.0.1"},"reference-count":11,"publisher":"S. Karger AG","issue":"6","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respiration"],"published-print":{"date-parts":[[2021]]},"abstract":"<b><i>Background:<\/i><\/b> Diagnosis, staging, and molecular profiling of lung cancer are mostly carried out with bronchoscopy or CT-guided aspiration\/biopsy. However, patients with locally advanced or advanced disease often harbor \u201csuperficial\u201d metastases for which a percutaneous, ultrasound-assisted needle aspiration\/biopsy (US-NAB) might represent an equally effective yet less invasive and costly alternative. <b><i>Patients and Methods:<\/i><\/b> We reviewed a prospectively collected database of consecutive patients with known\/suspected lung cancer who underwent a US-NAB of a suspected \u201csuperficial\u201d metastasis. Cancer genotyping was carried out with next-generation sequencing using the Oncomine\u2122 Focus DNA and RNA fusion panels. PD-L1 immunohistochemistry was performed with the SP263 antibody. Feasibility, diagnostic yield for tissue diagnosis, sensitivity for malignancy, diagnostic yield for the molecular profiling, and complications were the study endpoints. <b><i>Results:<\/i><\/b> A total of 98 lesions were evaluated, and 93 were biopsied (95% feasibility). The spectrum of sampled sites included lymph nodes (63 patients), bone (11), subcutaneous tissue (8), muscle (7), and the pleura (4). The diagnostic yield for a tissue diagnosis was 93% (91\/98). US-NAB correctly identified 85 of the 87 patients finally diagnosed with malignancy (98% sensitivity). Cancer genotyping and PDL1 testing were successfully completed in 41\/42 patients (98%) and in 40\/50 patients (80%) for whom these tests were requested, respectively. No complications were observed. <b><i>Conclusion:<\/i><\/b> US-NAB of \u201csuperficial\u201d metastasis of lung cancer is safe and is associated with high success for diagnosis and molecular profiling. In this clinical setting, using US-NAB as a first-step technique would significantly limit the use of more invasive and costly diagnostic procedures. <\/jats:p>","DOI":"10.1159\/000514316","type":"journal-article","created":{"date-parts":[[2021,4,7]],"date-time":"2021-04-07T21:01:10Z","timestamp":1617829270000},"page":"515-522","source":"Crossref","is-referenced-by-count":15,"title":["Diagnosis and Molecular Profiling of Lung Cancer by Percutaneous Ultrasound-Guided Biopsy of Superficial Metastatic Sites Is Safe and Highly Effective"],"prefix":"10.1159","volume":"100","author":[{"given":"Vanina","family":"Livi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Paioli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Cancellieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1386-4660","authenticated-orcid":false,"given":"Sara","family":"Betti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Natali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Ferrari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelangelo","family":"Fiorentino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1067-4696","authenticated-orcid":false,"given":"Rocco","family":"Trisolini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2021,4,7]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soc.2019.08.002"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2020.05.002"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2020.06.006"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdy275"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1159\/000362930"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1159\/000080638"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1007\/s00330-016-4357-8"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2018.11.013"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1378\/chest.12-0350"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cllc.2017.11.010"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1159\/000495661"}],"container-title":["Respiration"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/514316","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/514316","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:23:22Z","timestamp":1745425402000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000514316"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":11,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6,3]]},"published-print":{"date-parts":[[2021,6,7]]}},"URL":"https:\/\/doi.org\/10.1159\/000514316","archive":["Portico"],"relation":{},"ISSN":["0025-7931","1423-0356"],"issn-type":[{"value":"0025-7931","type":"print"},{"value":"1423-0356","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021]]}}}