{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T20:01:57Z","timestamp":1782763317087,"version":"3.54.5"},"reference-count":29,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Skin Pharmacol Physiol"],"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p>&lt;b&gt;&lt;i&gt;Introduction:&lt;\/i&gt;&lt;\/b&gt; This study investigated serum miR-221-3p levels in psoriatic patients and the characterization of serum miR-221-3p in keratinocyte inflammatory responses was further assessed. &lt;b&gt;&lt;i&gt;Methods:&lt;\/i&gt;&lt;\/b&gt; qRT-PCR was used to detect the expression level of miR-221-3p in the serum of 46 patients with psoriasis and 42 healthy controls. The receiver operating characteristic curve evaluated the diagnostic ability of miR-221-3p in psoriasis. The effect of miR-221-3p on HaCaT cell proliferation was detected by using a cell counting Kit-8 and Transwell. ELISA was used to detect serum and keratinocyte pro-inflammatory factors. &lt;b&gt;&lt;i&gt;Results:&lt;\/i&gt;&lt;\/b&gt; miR-221-3p was significantly increased in the serum of patients with psoriasis. The area under the curve was 0.861, the sensitivity was 80.4%, and the specificity was 85.7%. Serum miR-221-3p was positively correlated with the expression levels of tumor necrosis factor-\u03b1, interleukin (IL)-17A, and IL-22. Cell experiments showed that reducing the expression of miR-221-3p could significantly inhibit cell proliferation. Additionally, miR-221-3p downregulation also inhibited the release of some inflammatory factors in the HaCaT cells. &lt;b&gt;&lt;i&gt;Discussion\/Conclusion:&lt;\/i&gt;&lt;\/b&gt; MiR-221-3p is a latent biomarker of psoriasis patients. Lower expression of miR-221-3p inhibits the cell proliferation and inflammatory responses of HaCaT cells, which offers a possible target for the therapeutic interventions of psoriasis. <\/jats:p>","DOI":"10.1159\/000515114","type":"journal-article","created":{"date-parts":[[2021,6,4]],"date-time":"2021-06-04T21:01:41Z","timestamp":1622840501000},"page":"300-306","source":"Crossref","is-referenced-by-count":15,"title":["MiR-221-3p as a Potential Biomarker for Patients with Psoriasis and Its Role in Inflammatory Responses in Keratinocytes"],"prefix":"10.1159","volume":"34","author":[{"given":"Zhichao","family":"Meng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwei","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2021,6,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22284-y"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2011.10497.x"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2006.06.021"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2013.08.010"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2013.07.013"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7435621"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.15283\/ijsc18146"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1177\/039463200902200423"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.106689"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2014.10.009"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2018.01.014"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1453"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.40"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-015-0435-y"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-2011"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2020.101573"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000610"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1042\/bsr20190867"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-21193-4"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902464"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1590\/2359-3997000000255"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1136\/bcr-2017-220088"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.03087"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13249"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1102224"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100501"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.8470"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/968549"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2013.1416"}],"container-title":["Skin Pharmacology and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/515114","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/515114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:30:28Z","timestamp":1745425828000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000515114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":29,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,9,1]]},"published-print":{"date-parts":[[2021,9,7]]}},"URL":"https:\/\/doi.org\/10.1159\/000515114","archive":["Portico"],"relation":{},"ISSN":["1660-5527","1660-5535"],"issn-type":[{"value":"1660-5527","type":"print"},{"value":"1660-5535","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021]]}}}