{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T03:39:05Z","timestamp":1785901145418,"version":"3.56.0"},"reference-count":46,"publisher":"S. Karger AG","issue":"5","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fetal Diagn Ther"],"published-print":{"date-parts":[[2021]]},"abstract":"<b><i>Purpose:<\/i><\/b> We examined select pulmonary effects and donor cell kinetics after transamniotic stem cell therapy (TRASCET) in a model of congenital diaphragmatic hernia (CDH). <b><i>Methods:<\/i><\/b> Pregnant dams (<i>n<\/i> = 58) received nitrofen on gestational day 9.5 (E9) to induce fetal CDH. Fetuses (<i>n<\/i> = 681) were divided into 4 groups: untreated (<i>n<\/i> = 99) and 3 groups receiving volume-matched intra-amniotic injections on E17 of either saline (<i>n<\/i> = 142), luciferase-labeled amniotic fluid-derived mesenchymal stem cells (afMSCs; <i>n<\/i> = 299), or acellular recombinant luciferase (<i>n<\/i> = 141). Pulmonary morphometry, quantitative gene expression of pulmonary vascular tone mediators, or screening for labeled afMSCs were performed at term (E22). Statistical comparisons were by Mann-Whitney U-test, nested ANOVA, and Wald test. <b><i>Results:<\/i><\/b> TRASCET led to significant downregulation of endothelial nitric oxide synthase and endothelin receptor-A expressions compared to both untreated and saline groups (both <i>p<\/i> &#x3c; 0.001). TRASCET also led to a significant decrease in arteriole wall thickness compared to the untreated group (<i>p<\/i> &#x3c; 0.001) but not the saline group (<i>p<\/i> = 0.180). Donor afMSCs were identified in the bone marrow and umbilical cord (<i>p<\/i> = 0.035 and 0.015, respectively, vs. plain luciferase controls). <b><i>Conclusions:<\/i><\/b> The effects of TRASCET in experimental CDH appear to be centered on the pulmonary vasculature and to derive from circulating donor cells. <\/jats:p>","DOI":"10.1159\/000515277","type":"journal-article","created":{"date-parts":[[2021,4,14]],"date-time":"2021-04-14T21:01:06Z","timestamp":1618434066000},"page":"381-391","source":"Crossref","is-referenced-by-count":11,"title":["Transamniotic Stem Cell Therapy for Experimental Congenital Diaphragmatic Hernia: Structural, Transcriptional, and Cell Kinetics Analyses in the Nitrofen Model"],"prefix":"10.1159","volume":"48","author":[{"given":"Alexander V.","family":"Chalphin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie 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