{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T21:28:12Z","timestamp":1760909292263,"version":"3.40.5"},"reference-count":19,"publisher":"S. Karger AG","issue":"3","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gynecol Obstet Invest"],"published-print":{"date-parts":[[2021]]},"abstract":"<b><i>Objective:<\/i><\/b> Our main objective was to assess the association between the markers p16 and Ki-67 and recurrence of disease in patients previously treated for cervical high-grade squamous intraepithelial lesion (HSIL). <b><i>Design:<\/i><\/b> This is a case-control study at the National Cancer Institute conducted between 2005 and 2015. Of the patients with a pathologically confirmed diagnosis of HSIL, 107 cases were selected. They were divided into 2 groups: 28 cases with recurrence after treatment and a control group of 79 patients without recurrence. We identified clinical, pathological, and treatment variables. <b><i>Methods:<\/i><\/b> Two experienced pathologists performed immunohistochemical analysis of biomarkers; they agreed on their interpretation, and we calculated the odds ratios (ORs) associated with recurrence. For group comparisons, we used the Wilcoxon signed-rank, \u03c7<sup>2<\/sup>, or Fisher\u2019s exact test, depending on the type of variable. We conducted logistic regression models to estimate ORs and determine the factors associated with recurrence. The recurrence-free period was defined as the time frame between conization and either recurrence of disease or the last date the patient was seen. We used Kaplan-Meier plots to visualize survival curves and log-rank tests to compare the curves. We established a <i>p<\/i> value &#x3c;0.05 as statistically significant. <b><i>Results:<\/i><\/b> After pathologists performed immunohistochemical analysis, they achieved an agreement level of 83.7% for p16 and 60% for Ki-67. We did not find an association between recurrence and either p16 expression (<i>p<\/i> = 0.69) or the percentage of Ki-67 expression (<i>p<\/i> = 0.71). The recurrence-free period analysis did not reveal a difference in p16 expression (<i>p<\/i> = 0.57) nor in the percentage of Ki-67 expression in the 3-tiered scale (<i>p<\/i> = 0.56). <b><i>Limitations:<\/i><\/b> Our main limitation was a reduced sample size. <b><i>Conclusion:<\/i><\/b> We found no association between p16 and Ki-67 positivity and the risk of recurrence in previously treated HSIL. <\/jats:p>","DOI":"10.1159\/000515894","type":"journal-article","created":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T21:01:46Z","timestamp":1623358906000},"page":"293-298","source":"Crossref","is-referenced-by-count":2,"title":["Association of p16 and Ki-67 with Risk of Recurrence in Previously Treated Cervical High-Grade Squamous Intraepithelial Lesions"],"prefix":"10.1159","volume":"86","author":[{"given":"Carlos Germ\u00e1n","family":"Arredondo-G\u00e1lvez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise","family":"Acu\u00f1a-Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Cant\u00fa-de-Le\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Gregorio","family":"Chanona-Vilchis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Avil\u00e9s-Salas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aar\u00f3n","family":"Gonz\u00e1lez-Enciso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3991-2253","authenticated-orcid":false,"given":"Antonio","family":"Bandala-Jaques","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3950-157X","authenticated-orcid":false,"given":"Salim Abraham","family":"Barquet-Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"127","published-online":{"date-parts":[[2021,6,10]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61416-0"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2012.12.027"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1097\/00001703-199802000-00009"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2011.11.023"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijgo.2012.02.017"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.5772\/31209"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/289315"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1309\/AJCP1LLX8QMDXHHO"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.patol.2011.12.003"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0b013e31817e9ac5"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPXSVCDZ3D5MZM"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(12)70529-6"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.3802\/jgo.2008.19.3.162"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2006.07.043"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0000000000000061"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2006.01.033"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1590\/0100-69912016001008"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1598643"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3181e8b2c4"}],"container-title":["Gynecologic and Obstetric Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/515894","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/515894","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T16:39:10Z","timestamp":1745426350000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000515894"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":19,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,7,20]]},"published-print":{"date-parts":[[2021,9,10]]}},"URL":"https:\/\/doi.org\/10.1159\/000515894","archive":["Portico"],"relation":{},"ISSN":["0378-7346","1423-002X"],"issn-type":[{"type":"print","value":"0378-7346"},{"type":"electronic","value":"1423-002X"}],"subject":[],"published":{"date-parts":[[2021]]}}}