{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:28:19Z","timestamp":1790627299928,"version":"4.1.0"},"reference-count":17,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T00:00:00Z","timestamp":1622592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T00:00:00Z","timestamp":1622592000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dermatology"],"published-print":{"date-parts":[[2022]]},"abstract":"<b><i>Background:<\/i><\/b> Hidradenitis suppurativa\/acne inversa (HS) is a chronic, recurrent inflammatory skin disease. Its pivotal pathogenetic event is believed to be the occlusion of the hair follicle generating a perifollicular lympho-histiocytic inflammation. However, knowledge of the exact HS pathogenesis requires further research. <b><i>Objective:<\/i><\/b> To develop a human HS model applicable in preclinical research which could help to understand the pathophysiology of HS and to determine the action of therapeutic candidates. <b><i>Methods:<\/i><\/b> The 3D-SeboSkin technology was applied to maintain explants of involved and uninvolved skin of HS patients ex vivo for 3 days. Detection of differential expression of previously detected HS biomarkers was performed by immunohistochemistry in a group of female patients (<i>n<\/i> = 9, mean age 37.2 \u00b1 8.4 years). <b><i>Results:<\/i><\/b> The application of the 3D-SeboSkin model preserved the structural integrity of lesional and perilesional HS skin ex vivo, as previously described for healthy skin. Moreover, the HS 3D-SeboSkin setting maintained the differential expression and pattern of several HS biomarkers (S100A9, KRT16, SERPINB3) in epidermal and dermal tissue and the appendages. <b><i>Conclusion:<\/i><\/b> We have validated HS 3D-SeboSkin as a reproducible, human model, which is appropriate for preclinical lesional and perilesional HS skin studies ex vivo.<\/jats:p>","DOI":"10.1159\/000515955","type":"journal-article","created":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T17:03:04Z","timestamp":1622653384000},"page":"236-243","source":"Crossref","is-referenced-by-count":12,"title":["3D-SeboSkin Model for Human ex vivo Studies of Hidradenitis Suppurativa\/Acne Inversa"],"prefix":"10.1159","volume":"238","author":[{"given":"Xiaoxiao","family":"Hou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir M.","family":"Hossini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios","family":"Nikolakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ottfried","family":"Balthasar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Kurtz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1646-2608","authenticated-orcid":false,"given":"Christos C.","family":"Zouboulis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"127","published-online":{"date-parts":[[2021,6,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2008.00712_1.x"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.4161\/derm.2.1.12490"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.18173"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12712"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.16147"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00771.x"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.102180999"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1111\/exd.13919"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-019-02868-4"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s11684-019-0722-8"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2011.10224.x"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.16264"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2018.10.021"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.3390\/cells8080807"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.353"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.2000.1683"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200508064"}],"container-title":["Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/515955","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.karger.com\/Article\/Pdf\/515955","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T12:39:36Z","timestamp":1745411976000},"score":1,"resource":{"primary":{"URL":"https:\/\/karger.com\/article\/doi\/10.1159\/000515955"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,2]]},"references-count":17,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,3,10]]},"published-print":{"date-parts":[[2022,3,14]]}},"URL":"https:\/\/doi.org\/10.1159\/000515955","archive":["Portico"],"relation":{},"ISSN":["1018-8665","1421-9832"],"issn-type":[{"value":"1018-8665","type":"print"},{"value":"1421-9832","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,2]]}}}