{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T20:31:07Z","timestamp":1788813067783,"version":"build-2803163510"},"reference-count":42,"publisher":"S. Karger AG","issue":"2","license":[{"start":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T00:00:00Z","timestamp":1619136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"},{"start":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T00:00:00Z","timestamp":1619136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.karger.com\/Services\/SiteLicenses"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dig Dis"],"published-print":{"date-parts":[[2022]]},"abstract":"<b><i>Aim:<\/i><\/b> The aim of this study was to study the role of key epigenetic regulators pertaining to DNA methylation and histone-modification systems in <i>Helicobacter pylori<\/i> (HP)-associated gastritis and gastric carcinogenesis. <b><i>Methods:<\/i><\/b> The expression of DNA methyltransferase (DNMT-1, 3A, and 3B) and the catalytic subunit of polycomb repressive complex-2 (enhancer of zeste homolog 2 [EZH2]) in gastric carcinomas (<i>n<\/i> = 104), mucosa adjacent to carcinoma (<i>n<\/i> = 104), HP-associated gastritis (<i>n<\/i> = 95), and histologically normal mucosa (<i>n<\/i> = 31) was assessed by immunohistochemistry and qRT-PCR. <b><i>Results:<\/i><\/b> The expression of all 3 DNMTs and EZH2 was significantly higher in HP-associated gastritis and carcinoma cases than in those with adjacent and normal mucosa. The expression of DNMT-1 and 3B was maximum in HP-associated gastritis. DNMT-3A showed higher expression in carcinoma-adjacent mucosa than in normal mucosa. Interestingly, the expression of EZH2 was higher in cases of HP-associated gastritis with metaplasia than in those without metaplasia and also in cases of intestinal type of adenocarcinoma. Significant positive correlation of EZH2 was identified with DNMT-1, DNMT-3A, and DNMT-3B. However, none of these markers was associated with survival outcome. <b><i>Conclusion:<\/i><\/b> This study establishes an important role of the key epigenetic regulators in the pathogenesis of both HP-associated gastritis and gastric carcinoma. Higher expression of all the epigenetic markers in the gastritis and their persistence in the carcinoma point toward their implications in HP-driven gastric carcinogenesis. Further, an inter-relation between the 2 arms of epigenetics, namely, DNA methylation and histone-modification in the pathogenesis of gastric carcinoma, is also documented. Given the reversibility of epigenetic phenomenon, these molecules may be of important therapeutic use.<\/jats:p>","DOI":"10.1159\/000516478","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T17:04:34Z","timestamp":1619197474000},"page":"156-167","source":"Crossref","is-referenced-by-count":12,"title":["Elevated Expression of DNA Methyltransferases and Enhancer of Zeste Homolog 2 in <b><i>Helicobacter pylori<\/i><\/b> - Gastritis and Gastric Carcinoma"],"prefix":"10.1159","volume":"40","author":[{"given":"Suvendu","family":"Purkait","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susama","family":"Patra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suvradeep","family":"Mitra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minakshi 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